Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 94: 2534-2544, 2003. First published January 31, 2003; doi:10.1152/japplphysiol.01042.2002
8750-7587/03 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
94/6/2534    most recent
01042.2002v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kozak, W.
Right arrow Articles by Kozak, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kozak, W.
Right arrow Articles by Kozak, A.
Vol. 94, Issue 6, 2534-2544, June 2003

HIGHLIGHTED TOPICS
Genetic Models in Applied Physiology
Selected Contribution: Differential role of nitric oxide synthase isoforms in fever of different etiologies: studies using Nos gene-deficient mice

Wieslaw Kozak1,2 and Anna Kozak1

1 Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912 and 2 Institute of General and Molecular Biology, University of Nicolaus Copernicus, 87-100 Torun, Poland

Male C57BL/6J mice deficient in nitric oxide synthase (NOS) genes (knockout) and control (wild-type) mice were implanted intra-abdominally with battery-operated miniature biotelemeters (model VMFH MiniMitter, Sunriver, OR) to monitor changes in body temperature. Intravenous injection of lipopolysaccharide (LPS; 50 µg/kg) was used to trigger fever in response to systemic inflammation in mice. To induce a febrile response to localized inflammation, the mice were injected subcutaneously with pure turpentine oil (30 µl/animal) into the left hindlimb. Oral administration (gavage) of NG-monomethyl-L-arginine (L-NMMA) for 3 days (80 mg · kg-1 · day-1 in corn oil) before injection of pyrogens was used to inhibit all three NOSs (NG-monomethyl-D-arginine acetate salt and corn oil were used as control). In normal male C57BL/6J mice, L-NMMA inhibited the LPS-induced fever by ~60%, whereas it augmented fever by ~65% in mice injected with turpentine. Challenging the respective NOS knockout mice with LPS and with L-NMMA revealed that inducible NOS and neuronal NOS isoforms are responsible for the induction of fever to LPS, whereas endothelial NOS (eNOS) is not involved. In contrast, none of the NOS isoforms appeared to trigger fever to turpentine. Inhibition of eNOS, however, exacerbates fever in mice treated with L-NMMA and turpentine, indicating that eNOS participates in the antipyretic mechanism. These data support the hypothesis that nitric oxide is a regulator of fever. Its action differs, however, depending on the pyrogen used and the NOS isoform.

biotelemetry; body temperature; endotoxin; turpentine; fever mechanism; nitric oxide; gene knockout mice


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Feleder, V. Perlik, and C. M. Blatteis
Preoptic nitric oxide attenuates endotoxic fever in guinea pigs by inhibiting the POA release of norepinephrine
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2007; 293(3): R1144 - R1151.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Y. Rudaya, A. A. Steiner, J. R. Robbins, A. S. Dragic, and A. A. Romanovsky
Thermoregulatory responses to lipopolysaccharide in the mouse: dependence on the dose and ambient temperature
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2005; 289(5): R1244 - R1252.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. B. Dias, M. C. Almeida, E. C. Carnio, and L. G. S. Branco
Role of nitric oxide in tolerance to lipopolysaccharide in mice
J Appl Physiol, April 1, 2005; 98(4): 1322 - 1327.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.