Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 94: 1910-1916, 2003. First published January 10, 2003; doi:10.1152/japplphysiol.00977.2002
8750-7587/03 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
94/5/1910    most recent
00977.2002v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Derave, W.
Right arrow Articles by Hespel, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Derave, W.
Right arrow Articles by Hespel, P.
Vol. 94, Issue 5, 1910-1916, May 2003

Combined creatine and protein supplementation in conjunction with resistance training promotes muscle GLUT-4 content and glucose tolerance in humans

Wim Derave1, Bert O. Eijnde1, Patricia Verbessem1, Monique Ramaekers1, Mark Van Leemputte1, Erik A. Richter2, and Peter Hespel1

1 Exercise Physiology and Biomechanics Laboratory, Department of Kinesiology, Faculty of Physical Education and Physiotherapy, Katholieke Universiteit Leuven, 3001 Leuven, Belgium; and 2 Copenhagen Muscle Research Centre, Institute of Exercise and Sports Sciences, University of Copenhagen, DK-2100 Copenhagen, Denmark

The present study was undertaken to explore the effects of creatine and creatine plus protein supplementation on GLUT-4 and glycogen content of human skeletal muscle. This was investigated in muscles undergoing a decrease (immobilization) and subsequent increase (resistance training) in activity level, compared with muscles with unaltered activity pattern. A double-blind, placebo-controlled trial was performed by 33 young healthy subjects. The subjects' right legs were immobilized with a cast for 2 wk, followed by a 6-wk resistance training program for the right knee extensor muscles. The participants were supplemented throughout the study with either placebo (Pl group) or creatine (Cr group) or with creatine during immobilization and creatine plus protein during retraining (Cr+P group). Needle biopsies were bilaterally taken from the vastus lateralis. GLUT-4 protein expression was reduced by the immobilization in all groups (P < 0.05). During retraining, GLUT-4 content increased (P < 0.05) in both Cr (+24%) and Cr+P (+33%), which resulted in higher posttraining GLUT-4 expression compared with Pl (P < 0.05). Compared with Pl, muscle glycogen content was higher (P < 0.05) in the trained leg in both Cr and Cr+P. Supplements had no effect on GLUT-4 expression or glycogen content in contralateral control legs. Area under the glucose curve during the oral glucose tolerance test was decreased from 232 ± 23 mmol · l-1 · min-1 at baseline to 170 ± 23 mmol · l-1 · min-1 at the end of the retraining period in Cr+P (P < 0.05), but it did not change in Cr or Pl. We conclude that creatine intake stimulates GLUT-4 and glycogen content in human muscle only when combined with changes in habitual activity level. Furthermore, combined protein and creatine supplementation improved oral glucose tolerance, which is supposedly unrelated to the changes in muscle GLUT-4 expression.

immobilization; skeletal muscle; glycogen


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
D. A. Sewell, T. M. Robinson, and P. L. Greenhaff
Creatine supplementation does not affect human skeletal muscle glycogen content in the absence of prior exercise
J Appl Physiol, February 1, 2008; 104(2): 508 - 512.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
W. Derave, N. Straumann, R. A. Olek, and P. Hespel
Electrolysis stimulates creatine transport and transporter cell surface expression in incubated mouse skeletal muscle: potential role of ROS
Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1250 - E1257.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
C. M. Ferrara, A. P. Goldberg, H. K. Ortmeyer, and A. S. Ryan
Effects of aerobic and resistive exercise training on glucose disposal and skeletal muscle metabolism in older men.
J. Gerontol. A Biol. Sci. Med. Sci., May 1, 2006; 61(5): 480 - 487.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J.-S. Ju, J. L. Smith, P. J. Oppelt, and J. S. Fisher
Creatine feeding increases GLUT4 expression in rat skeletal muscle
Am J Physiol Endocrinol Metab, February 1, 2005; 288(2): E347 - E352.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. M. Murphy, D. G. Stephenson, and G. D. Lamb
Effect of creatine on contractile force and sensitivity in mechanically skinned single fibers from rat skeletal muscle
Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1589 - C1595.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. K. Holten, M. Zacho, M. Gaster, C. Juel, J. F.P. Wojtaszewski, and F. Dela
Strength Training Increases Insulin-Mediated Glucose Uptake, GLUT4 Content, and Insulin Signaling in Skeletal Muscle in Patients With Type 2 Diabetes
Diabetes, February 1, 2004; 53(2): 294 - 305.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online