Vol. 94, Issue 5, 1870-1874, May 2003
Na+-K+-ATPase 
2-gene and skeletal
muscle characteristics in response to long-term overfeeding
Olavi
Ukkola1,2,
Denis R.
Joanisse3,
Angelo
Tremblay3, and
Claude
Bouchard1
1 Pennington Biomedical Research
Center, Baton Rouge, Louisiana 70808-4124;
2 Department of Internal Medicine and
Biocenter Oulu, University of Oulu, Oulu, Finland
FIN-90220; and 3 Physical Activity Sciences
Laboratory, Division of Kinesiology, Laval University, Ste-Foy,
Québec, Canada G1V 4G2
The role
of Na+-K+-ATPase 
2-gene BglII
polymorphism in the changes of skeletal muscle metabolic properties
after a 100-day overfeeding protocol conducted with 12 pairs of
monozygotic twins is reported. The activities of oxoglutarate
dehydrogenase (OGDH) and phosphofructokinase (PFK) were determined from
muscle biopsies. A larger increase in the total fat mass (127 vs. 61%)
(P < 0.05) and low-density lipoprotein cholesterol (20 vs. 0.7%) (P = 0.05) in 8.0/8.0-kb [3.3-kb negative
(
); n = 7 pairs] than in 8.0/3.3 + 3.3/3.3-kb
[3.3-kb positive (+); n = 5 pairs] subjects was
observed. OGDH activity decreased in 3.3-kb
(15%),
whereas PFK (+26%) as well as the PFK-to-OGDH ratio (90%) increased.
In contrast, among 3.3-kb+, OGDH increased (+54%) together
with a decrease in PFK (1%) and PFK-to-OGDH ratio (5%). These
changes were significantly different between genotypes (P
from <0.05 to 0.01). In conclusion, fat mass, low-density lipoprotein
cholesterol, and skeletal muscle glycolytic-to-oxidative enzyme
ratio increased more in the 2-gene 3.3-kb subjects
with overfeeding, suggesting more unfavorable metabolic changes
compared with the 3.3-kb+ subjects.
genes; glycolysis; oxidation; lipids; abdominal obesity
