Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 94: 1403-1409, 2003; doi:10.1152/japplphysiol.00363.2002
8750-7587/03 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cogo, A.
Right arrow Articles by Martin, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cogo, A.
Right arrow Articles by Martin, J. G.
Vol. 94, Issue 4, 1403-1409, April 2003

Effects of hypoxia on rat airway smooth muscle cell proliferation

A. Cogo1, G. Napolitano2, M. C. Michoud2, D. Ramos Barbon2, M. Ward2, and J. G. Martin2

2 Meakins-Christie Laboratories and Montreal Chest Institute Research Center, McGill University, Montréal, Québec, Canada H2X 2P2; and 1 Department of Clinical and Experimental Medicine, University of Ferrara, 44100 Ferrara, Italy

Although it is well known that hypoxemia induces pulmonary vasoconstriction and vascular remodeling, due to the proliferation of both vascular smooth muscle cells and fibroblasts, the effects of hypoxemia on airway smooth muscle cells are not well characterized. The present study was designed to assess the in vitro effects of hypoxia (1 or 3% O2) on rat airway smooth muscle cell growth and response to mitogens (PDGF and 5-HT). Cell growth was assessed by cell counting and cell cycle analysis. Compared with normoxia (21% O2), there was a 42.2% increase in the rate of proliferation of cells exposed to 3% O2 (72 h, P = 0.006), as well as an enhanced response to PDGF (13.9% increase; P = 0.023) and to 5-HT (17.2% increase; P = 0.039). Exposure to 1% O2 (72 h) decreased cell proliferation by 21.0% (P = 0.017) and reduced the increase in cell proliferation induced by PGDF and 5-HT by 16.2 and 15.7%, respectively (P = 0.019 and P = 0.011). A significant inhibition in hypoxia-induced cell proliferation was observed after the administration of bisindolylmaleimide GF-109203X (a specific PKC inhibitor) or downregulation of PKC with PMA. Pretreatment with GF-109203X decreased proliferation by 21.5% (P = 0.004) and PMA by 31.5% (P = 0.005). These results show that hypoxia induces airway smooth muscle cell proliferation, which is at least partially dependent on PKC activation. They suggest that hypoxia could contribute to airway remodeling in patients suffering from chronic, severe respiratory diseases.

hypoxemia; protein kinase C; cell cycle; in vitro; cell growth


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. B. Ray, S. Arab, Y. Deng, P. Liu, L. Penn, D. W. Courtman, and M. E. Ward
Oxygen regulation of arterial smooth muscle cell proliferation and survival
Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H839 - H852.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. Chachami, A. Hatziefthimiou, P. Liakos, M. G. Ioannou, G. K. Koukoulis, S. Bonanou, P.-A. Molyvdas, G. Simos, and E. Paraskeva
Exposure of differentiated airway smooth muscle cells to serum stimulates both induction of hypoxia-inducible factor-1{alpha} and airway responsiveness to ACh
Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L913 - L922.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. R. Preston, N. S. Hill, R. R. Warburton, and B. L. Fanburg
Role of 12-lipoxygenase in hypoxia-induced rat pulmonary artery smooth muscle cell proliferation
Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L367 - L374.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
E. V. Gerasimovskaya, D. A. Tucker, and K. R. Stenmark
Activation of phosphatidylinositol 3-kinase, Akt, and mammalian target of rapamycin is necessary for hypoxia-induced pulmonary artery adventitial fibroblast proliferation
J Appl Physiol, February 1, 2005; 98(2): 722 - 731.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Chachami, G. Simos, A. Hatziefthimiou, S. Bonanou, P.-A. Molyvdas, and E. Paraskeva
Cobalt Induces Hypoxia-Inducible Factor-1{alpha} Expression in Airway Smooth Muscle Cells by a Reactive Oxygen Species- and PI3K-Dependent Mechanism
Am. J. Respir. Cell Mol. Biol., November 1, 2004; 31(5): 544 - 551.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online