Journal of Applied Physiology Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 94: 1365-1372, 2003. First published November 27, 2002; doi:10.1152/japplphysiol.00833.2002
8750-7587/03 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
94/4/1365    most recent
00833.2002v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johnson, N. A.
Right arrow Articles by Thompson, M. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johnson, N. A.
Right arrow Articles by Thompson, M. W.
Vol. 94, Issue 4, 1365-1372, April 2003

Intramyocellular triacylglycerol in prolonged cycling with high- and low-carbohydrate availability

N. A. Johnson1, S. R. Stannard1, K. Mehalski2, M. I. Trenell2, T. Sachinwalla3, C. H. Thompson2, and M. W. Thompson1

1 School of Exercise and Sport Science, The University of Sydney, Lidcombe 1825, New South Wales; 2 School of Molecular and Microbial Bioscience, The University of Sydney, Sydney 2006, New South Wales; and 3 Rayscan Imaging, Liverpool 2170, New South Wales, Australia

Vastus lateralis intramyocellular lipid (IMCL) content was assessed by 1H-magnetic resonance spectroscopy before and after prolonged time trial cycling bouts of ~3-h duration. Six highly trained male cyclists completed a double-blind, randomized, crossover design of two experimental trials after a strenuous exercise bout and 48 h of high (HC) (9.32 ± 0.08 g · kg-1 · day-1) and low (LC) (0.59 ± 0.21 g · kg-1 · day-1) dietary carbohydrate. Resting IMCL content was significantly higher after LC vs. HC (P < 0.01) and was reduced during exercise by 64 and 57%, respectively. IMCL was not different between conditions after exercise (P > 0.05). The approximately twofold increase in IMCL degradation in LC compared with HC suggests that higher rates of whole body lipid metabolism in LC were in part attributable to a greater IMCL utilization. Four subjects experienced reductions of IMCL in excess of 70% during exercise. To our knowledge, this is the first study to report near depletion of IMCL during prolonged cycling, indicating that IMCL, presumably the triacylglycerol component, may be exhausted by prolonged strenuous exercise.

endurance exercise; intramyocellular lipid; proton magnetic resonance spectroscopy; carbohydrate loading; free fatty acids


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. Fernandez, O. Hansson, P. Nevsten, C. Holm, and C. Klint
Hormone-sensitive lipase is necessary for normal mobilization of lipids during submaximal exercise
Am J Physiol Endocrinol Metab, July 1, 2008; 295(1): E179 - E186.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. De Bock, T. Dresselaers, B. Kiens, E. A. Richter, P. Van Hecke, and P. Hespel
Evaluation of intramyocellular lipid breakdown during exercise by biochemical assay, NMR spectroscopy, and Oil Red O staining
Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E428 - E434.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
T. Stellingwerff, H. Boon, R. A. M. Jonkers, J. M. Senden, L. L. Spriet, R. Koopman, and L. J. C. van Loon
Significant intramyocellular lipid use during prolonged cycling in endurance-trained males as assessed by three different methodologies
Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1715 - E1723.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
N. A. Johnson, S. R. Stannard, D. S. Rowlands, P. G. Chapman, C. H. Thompson, H. O'Connor, T. Sachinwalla, and M. W. Thompson
Effect of short-term starvation versus high-fat diet on intramyocellular triglyceride accumulation and insulin resistance in physically fit men
Exp Physiol, July 1, 2006; 91(4): 693 - 703.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
B. Kiens
Skeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance
Physiol Rev, January 1, 2006; 86(1): 205 - 243.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
T. W. Zderic, S. Schenk, C. J. Davidson, L. O. Byerley, and E. F. Coyle
Manipulation of dietary carbohydrate and muscle glycogen affects glucose uptake during exercise when fat oxidation is impaired by {beta}-adrenergic blockade
Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1195 - E1201.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. J. C. van Loon
Use of intramuscular triacylglycerol as a substrate source during exercise in humans
J Appl Physiol, October 1, 2004; 97(4): 1170 - 1187.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. R. Stannard and N. A. Johnson
Insulin resistance and elevated triglyceride in muscle: more important for survival than 'thrifty' genes?
J. Physiol., February 1, 2004; 554(3): 595 - 607.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
T. W. Zderic, C. J. Davidson, S. Schenk, L. O. Byerley, and E. F. Coyle
High-fat diet elevates resting intramuscular triglyceride concentration and whole body lipolysis during exercise
Am J Physiol Endocrinol Metab, February 1, 2004; 286(2): E217 - E225.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
B. Schmitt, M. Fluck, J. Decombaz, R. Kreis, C. Boesch, M. Wittwer, F. Graber, M. Vogt, H. Howald, and H. Hoppeler
Transcriptional adaptations of lipid metabolism in tibialis anterior muscle of endurance-trained athletes
Physiol Genomics, October 17, 2003; 15(2): 148 - 157.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online