Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 94: 60-68, 2003. First published September 20, 2002; doi:10.1152/japplphysiol.00269.2002
8750-7587/03 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
94/1/60    most recent
00269.2002v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyauchi, T.
Right arrow Articles by Matsuda, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyauchi, T.
Right arrow Articles by Matsuda, M.
Vol. 94, Issue 1, 60-68, January 2003

Exercise causes a tissue-specific change of NO production in the kidney and lung

Takashi Miyauchi1, Seiji Maeda2, Motoyuki Iemitsu1, Tsutomu Kobayashi1, Yoshito Kumagai3, Iwao Yamaguchi1, and Mitsuo Matsuda2

1 Cardiovascular Division, Department of Internal Medicine, Institute of Clinical Medicine, 2 Department of Sports Medicine, Institute of Health and Sport Sciences, and 3 Department of Environmental Medicine, Institute of Community Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-0006, Japan

Nitric oxide (NO) is produced in the vascular endothelium and is a potent vasodilator substance that participates in the regulation of local vascular tone. Exercise causes peculiar changes in systemic and regional blood flow, i.e., an increase of systemic blood flow and a redistribution of local tissue blood flow, by which the blood flow is greatly increased in the working muscles, whereas it is decreased in some organs such as the kidney and intestine. Thus we hypothesized that exercise causes a tissue-specific change of NO production in some internal organs. We studied whether exercise affects expression of NO synthase (NOS) mRNA and protein, NOS activity, and tissue level of nitrite/nitrate (stable end products of NO) in the kidneys (in which blood flow during exercise is decreased) and lungs (in which blood flow during exercise is increased with the increase of cardiac output) of rat. Rats ran on a treadmill for 45 min at a speed of 25 m/min. Immediately after this exercise, kidneys and lungs were quickly removed. Control rats remained at rest during this 45-min period. Expression of endothelial NOS (eNOS) mRNA in the kidneys was markedly lower in exercise rats than in control rats, whereas that in the lungs was significantly higher in exercise rats than in control rats. Western blot analysis confirmed down- and upregulation of eNOS protein in the kidney and lung, respectively, after exercise. On the other hand, neither expression of neuronal NOS (nNOS) mRNA and nNOS protein nor inducible NOS (iNOS) mRNA and iNOS protein in the kidneys and lungs differed between exercise and control rats. NOS activity in the kidney was significantly lower in exercise rats than in control rats, whereas that in the lung was significantly higher in exercise rats than in control rats. On the other hand, the iNOS activity in the kidneys and lungs did not differ between exercise rats and control rats. Tissue nitrite/nitrate level in the kidneys was markedly lower in exercise rats, whereas that in the lungs was significantly higher in exercise rats. The present results show that production of NO is markedly and tissue-specifically changed in the kidney and lung by exercise.

treadmill running; nitric oxide synthase mRNA; tissue nitrite/nitrate level; nitric oxide synthase activity; redistribution of blood flow


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
O. Yalcin, P. Ulker, U. Yavuzer, H. J. Meiselman, and O. K. Baskurt
Nitric oxide generation by endothelial cells exposed to shear stress in glass tubes perfused with red blood cell suspensions: role of aggregation
Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2098 - H2105.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. D. Moraes, G. Gioseffi, A. C. L. Nobrega, and E. Tibirica
Effects of exercise training on the vascular reactivity of the whole kidney circulation in rabbits
J Appl Physiol, August 1, 2004; 97(2): 683 - 688.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. Maeda, T. Miyauchi, M. Iemitsu, T. Tanabe, K. Goto, I. Yamaguchi, and M. Matsuda
Endothelin receptor antagonist reverses decreased NO system in the kidney in vivo during exercise
Am J Physiol Endocrinol Metab, April 1, 2004; 286(4): E609 - E614.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. E. Davis, I. M. Grumbach, T. Fukai, A. Cutchins, and D. G. Harrison
Shear Stress Regulates Endothelial Nitric-oxide Synthase Promoter Activity through Nuclear Factor {kappa}B Binding
J. Biol. Chem., January 2, 2004; 279(1): 163 - 168.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
O. K. Baskurt, O. Yalcin, S. Ozdem, J. K. Armstrong, and H. J. Meiselman
Modulation of endothelial nitric oxide synthase expression by red blood cell aggregation
Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H222 - H229.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online