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J Appl Physiol 93: 2147-2154, 2002; doi:10.1152/japplphysiol.01134.2001
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Vol. 93, Issue 6, 2147-2154, December 2002

Validation of the DLW method in Japanese quail at different water fluxes using laser and IRMS

R. Van Trigt1, E. R. T. Kerstel1, R. E. M. Neubert1, H. A. J. Meijer1, M. McLean2, and G. H. Visser1,2

1 Centrum voor IsotopenOnderzoek, University of Groningen, 9747 AG Groningen; and 2 Zoological Laboratory, 9750 AA Haren, The Netherlands

In Japanese quail (Coturnix c. japonica; n = 9), the doubly labeled water (DLW) method (2H, 18O) for estimation of CO2 production (l/day) was validated. To evaluate its sensitivity to water efflux levels (rH2Oe; g/day) and to assumptions of fractional evaporative water loss (x; dimensionless), animals were repeatedly fed a dry pellet diet (average rH2Oe of 34.8 g/day) or a wet mash diet (95.8 g/day). We simultaneously compared the novel infrared laser spectrometry (LS) with isotope ratio mass spectrometry. At low rH2Oe, calculated CO2 production rate exhibited little sensitivity to assumptions concerning x, with the best fit being found at 0.51, and only little error was made employing an x value of 0.25. In contrast, at high rH2Oe, sensitivities were much higher with the best fit at x = 0.32. Conclusions derived from isotope ratio mass spectrometry and LS were similar, proving the usefulness of LS. Within a threefold range of rH2Oe, little error in the DLW method is made when assuming one single x value of 0.25 (recommended by Speakman JR, Doubly Labelled Water. Theory and Practice. London: Chapman & Hall, 1997), indicating its robustness in comparative studies.

isotope ratio mass spectrometry; laser spectrometry; doubly labeled water; energy expenditure; water flux


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