| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 2 Medicine and Cardiovascular Center and 1 Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226
In contrast to its constrictor
effects on peripheral arteries, 20-hydroxyeicosatetraenoic acid
(20-HETE) is an endothelial-dependent dilator of pulmonary arteries
(PAs). The present study examined the hypothesis that the vasodilator
effects of 20-HETE in PAs are due to an elevation of intracellular
calcium concentration ([Ca2+]i) and the
release of nitric oxide (NO) from bovine PA endothelial cells (BPAECs).
BPAECs express cytochrome P-450 4A (CYP4A) protein and
produce 20-HETE. 20-HETE dilated PAs preconstricted with U-46619 or
norepinephrine and treated with the cytochrome P-450
inhibitor 17-octadecynoic acid and the cyclooxygenase inhibitor
indomethacin. The dilator effect of 20-HETE was blocked by the NO
synthase inhibitor N
-nitro-L-arginine methyl ester
(L-NAME) or by removal of endothelium. 20-HETE
significantly increased [Ca2+]i and NO
production in BPAECs. 20-HETE-induced NO release was blunted by removal
of extracellular calcium, as well as NO synthase inhibitors
(L-NAME). These results suggest that 20-HETE dilates PAs at
least in part by increasing [Ca2+]i and NO
release in BPAECs.
cytochrome P-450; calcium transients; pulmonary artery endothelial cells; vasodilation; 20-hydroxyeicosatetraenoic acid
This article has been cited by other articles:
|
|
 |
|
  J. Cheng, J.-S. Ou, H. Singh, J. R. Falck, D. Narsimhaswamy, K. A. Pritchard Jr., and M. L. Schwartzman 20-Hydroxyeicosatetraenoic acid causes endothelial dysfunction via eNOS uncoupling Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H1018 - H1026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Paffett, J. S. Naik, T. C. Resta, and B. R. Walker Reduced store-operated Ca2+ entry in pulmonary endothelial cells from chronically hypoxic rats Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1135 - L1142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Fang, F. M. Faraci, T. L. Kaduce, S. Harmon, M. L. Modrick, S. Hu, S. A. Moore, J. R. Falck, N. L. Weintraub, and A. A. Spector 20-Hydroxyeicosatetraenoic acid is a potent dilator of mouse basilar artery: role of cyclooxygenase Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2301 - H2307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Jacobs, D. Zhu, S. Gruenloh, B. Lopez, and M. Medhora VEGF-induced relaxation of pulmonary arteries is mediated by endothelial cytochrome P-450 hydroxylase Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L369 - L377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, M. Medhora, J. R. Falck, K. A. Pritchard Jr, and E. R. Jacobs Mechanisms of activation of eNOS by 20-HETE and VEGF in bovine pulmonary artery endothelial cells Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L378 - L385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-S. Wang, H. Singh, F. Zhang, T. Ishizuka, H. Deng, R. Kemp, M. S. Wolin, T. H. Hintze, N. G. Abraham, A. Nasjletti, et al. Endothelial Dysfunction and Hypertension in Rats Transduced With CYP4A2 Adenovirus Circ. Res., April 14, 2006; 98(7): 962 - 969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Parker, T. R. Grover, J. P. Kinsella, J. R. Falck, and S. H. Abman Inhibition of 20-HETE abolishes the myogenic response during NOS antagonism in the ovine fetal pulmonary circulation Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L261 - L267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E Rousseau, M. Cloutier, C. Morin, and S. Proteau Capsazepine, a vanilloid antagonist, abolishes tonic responses induced by 20-HETE on guinea pig airway smooth muscle Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L460 - L470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Ljubimov and M. B. Grant P450 in the Angiogenesis Affair: The Unusual Suspect Am. J. Pathol., February 1, 2005; 166(2): 341 - 344. [Full Text] [PDF]
|
|
|
|
|
|
F. Mercier, C. Morin, M. Cloutier, S. Proteau, J. Rokach, W. S. Powell, and E. Rousseau 5-Oxo-ETE regulates tone of guinea pig airway smooth muscle via activation of Ca2+ pools and Rho-kinase pathway Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L631 - L640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fuloria, D. M. Eckman, D. A. Leach, and J. L. Aschner 20-Hydroxyeicosatetraenoic acid is a vasoconstrictor in the newborn piglet pulmonary microcirculation Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L360 - L365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhu, T. Mori, T. Huang, and J. H. Lombard Effect of high-salt diet on NO release and superoxide production in rat aorta Am J Physiol Heart Circ Physiol, February 1, 2004; 286(2): H575 - H583. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
