Journal of Applied Physiology Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 93: 1208-1214, 2002. First published May 10, 2002; doi:10.1152/japplphysiol.01121.2001
8750-7587/02 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
93/4/1208    most recent
01121.2001v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Glaab, T.
Right arrow Articles by Fabel, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Glaab, T.
Right arrow Articles by Fabel, H.
Vol. 93, Issue 4, 1208-1214, October 2002

Noninvasive measurement of midexpiratory flow indicates bronchoconstriction in allergic rats

Thomas Glaab1,*, Heinz G. Hoymann2,*, Jens M. Hohlfeld1,2, Regina Korolewitz2, Matthias Hecht2, Yves Alarie3, Thomas Tschernig4, Armin Braun2, Norbert Krug2, and Helmut Fabel1

Departments of 1 Respiratory Medicine and 4 Anatomy, Hannover Medical School, and 2 Fraunhofer Institute of Toxicology and Aerosol Research, Drug Research and Clinical Inhalation, 30625 Hannover, Germany; and 3 Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15238

This study was designed to evaluate the value and applicability of tidal breathing pattern analysis to assess bronchoconstriction in conscious rats. Using noninvasive, head-out body plethysmography and the decrease in tidal midexpiratory flow (EF50), we measured airway responsiveness (AR) to inhaled acetylcholine and allergen in conscious Brown-Norway rats, followed by invasive determination of pulmonary conductance (GL) and EF50 in anesthetized rats. Dose-response studies to acetylcholine showed that noninvasively recorded EF50 closely reflected the dose-dependent decreases observed with the invasive monitoring of simultaneously measured GL and EF50. After sensitization and intratracheal boost to ovalbumin or saline, rats were assessed for early and late AR to aerosolized ovalbumin. Ovalbumin aerosol challenge resulted in early and late AR in allergen-sensitized rats, whereas controls were unresponsive. The allergen-specific AR, as measured noninvasively by EF50, was similar in degree compared with invasively recorded EF50 and GL and was associated with enhanced IgE and airway inflammation. We conclude that EF50 is a noninvasive and physiologically valid index of bronchoconstriction in a rat model of asthma.

animal models; allergy; immunology; respiratory function test; asthma

* T. Glaab and H. G. Hoymann contributed equally to this work.




This article has been cited by other articles:


Home page
 Eur Respir JHome page
A. O. Yildirim, M. Veith, T. Rausch, B. Muller, P. Kilb, L. S. Van Winkle, and H. Fehrenbach
Keratinocyte growth factor protects against Clara cell injury induced by naphthalene
Eur. Respir. J., September 1, 2008; 32(3): 694 - 704.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Skripuletz, A. Schmiedl, J. Schade, S. Bedoui, T. Glaab, R. Pabst, S. von Horsten, and M. Stephan
Dose-dependent recruitment of CD25+ and CD26+ T cells in a novel F344 rat model of asthma
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1564 - L1571.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. L. S. Lofgren, M. R. Mazan, E. P. Ingenito, K. Lascola, M. Seavey, A. Walsh, and A. M. Hoffman
Restrained whole body plethysmography for measure of strain-specific and allergen-induced airway responsiveness in conscious mice
J Appl Physiol, November 1, 2006; 101(5): 1495 - 1505.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Glaab, W. Mitzner, A. Braun, H. Ernst, R. Korolewitz, J. M. Hohlfeld, N. Krug, and H. G. Hoymann
Repetitive measurements of pulmonary mechanics to inhaled cholinergic challenge in spontaneously breathing mice
J Appl Physiol, September 1, 2004; 97(3): 1104 - 1111.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. Adler, G. Cieslewicz, and C. G. Irvin
Unrestrained plethysmography is an unreliable measure of airway responsiveness in BALB/c and C57BL/6 mice
J Appl Physiol, July 1, 2004; 97(1): 286 - 292.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Z. Hantos and V. Brusasco
Assessment of respiratory mechanics in small animals: the simpler the better?
J Appl Physiol, October 1, 2002; 93(4): 1196 - 1197.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online