Journal of Applied Physiology AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 93: 1140-1151, 2002. First published May 31, 2002; doi:10.1152/japplphysiol.00307.2002
8750-7587/02 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow A corrigendum has been published
Right arrow All Versions of this Article:
93/3/1140    most recent
00307.2002v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (49)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, Y.
Right arrow Articles by Xiao, Y.-F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, Y.
Right arrow Articles by Xiao, Y.-F.
Vol. 93, Issue 3, 1140-1151, September 2002

VEGF enhances functional improvement of postinfarcted hearts by transplantation of ESC-differentiated cells

Yinke Yang, Jiang-Yong Min, Jamal S. Rana, Qingen Ke, Jingbo Cai, Yu Chen, James P. Morgan, and Yong-Fu Xiao

Stem Cell Research Laboratory, The Charles A. Dana Research Institute and Harvard-Thorndike Laboratory, Cardiovascular Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215

Despite considerable advances in medicine, the incidence of heart failure remains high in patients after myocardial infarction (MI). This study investigated the effects of engrafted early-differentiated cells (EDCs) from mouse embryonic stem cells, with or without transfection of vascular endothelial growth factor (VEGF) cDNA (phVEGF165), on cardiac function in postinfarcted mice. EDCs were transfected with green fluorescent protein (GFP) cDNA and transplanted into infarcted myocardium. Compared with the MI mice receiving cell-free medium, cardiac function was significantly improved in the MI mice 6 wk after transplantation of EDCs. Moreover, improvement of heart function was significantly greater in the mice implanted with EDCs overexpressing VEGF (EDCs-VEGF) than with EDCs alone. Frozen sections of infarcted myocardium with EDCs or EDCs-VEGF transplantation showed GFP-positive tissue. The area with positive immunostaining for cardiac troponin I and alpha -myosin heavy chain was larger in injured myocardium with EDCs or EDCs-VEGF transplantation than with medium injection. Transplantation of EDCs or EDCs-VEGF significantly increased the number of blood vessels in the MI area. However, the density of capillaries was significantly higher in the EDCs-VEGF animals than in the EDC mice. Double staining for GFP and connexin-43 was positive in injured myocardium with EDC transplantation. Our data demonstrate that engrafted EDCs or EDCs-VEGF regenerated cardiac tissue and significantly improved cardiac function in postinfarcted hearts. The novel EDCs-VEGF synergistic approach may have an important impact on future cell therapy for patients experiencing MI or heart failure.

embryonic stem cells; vascular endothelial growth factor; myocardial infarction; cell transplantation


This article has been cited by other articles:


Home page
 Circ Cardiovasc ImagingHome page
T.-C. Hung, Y. Suzuki, T. Urashima, A. Caffarelli, G. Hoyt, A. Y. Sheikh, A. C. Yeung, I. Weissman, R. C. Robbins, J. M. Bulte, et al.
Multimodality Evaluation of the Viability of Stem Cells Delivered Into Different Zones of Myocardial Infarction
Circ Cardiovasc Imaging, July 1, 2008; 1(1): 6 - 13.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. Nussbaum, E. Minami, M. A. Laflamme, J. A. I. Virag, C. B. Ware, A. Masino, V. Muskheli, L. Pabon, H. Reinecke, and C. E. Murry
Transplantation of undifferentiated murine embryonic stem cells in the heart: teratoma formation and immune response
FASEB J, May 1, 2007; 21(7): 1345 - 1357.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
V. Chintalgattu, G. S. Harris, S. M. Akula, and L. C. Katwa
PPAR-{gamma} agonists induce the expression of VEGF and its receptors in cultured cardiac myofibroblasts
Cardiovasc Res, April 1, 2007; 74(1): 140 - 150.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
M. A. Retuerto, J. T. Beckmann, J. Carbray, G. Patejunas, S. Sarateanu, B. J. Kane, B. Smulevitz, D. D. McPherson, and T. K. Rosengart
Angiogenic pretreatment to enhance myocardial function after cellular cardiomyoplasty with skeletal myoblasts
J. Thorac. Cardiovasc. Surg., February 1, 2007; 133(2): 478 - 484.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
L. W. van Laake, R. Hassink, P. A. Doevendans, and C. Mummery
Heart repair and stem cells
J. Physiol., December 1, 2006; 577(2): 467 - 478.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Chen, I. Amende, T. G. Hampton, Y. Yang, Q. Ke, J.-Y. Min, Y.-F. Xiao, and J. P. Morgan
Vascular endothelial growth factor promotes cardiomyocyte differentiation of embryonic stem cells
Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1653 - H1658.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
A. J. Boyle, S. P. Schulman, and J. M. Hare
Stem Cell Therapy for Cardiac Repair: Ready for the Next Step
Circulation, July 25, 2006; 114(4): 339 - 352.
[Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. Leri, J. Kajstura, and P. Anversa
Cardiac Stem Cells and Mechanisms of Myocardial Regeneration
Physiol Rev, October 1, 2005; 85(4): 1373 - 1416.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
K. Azarnoush, A. Maurel, L. Sebbah, C. Carrion, A. Bissery, C. Mandet, J. Pouly, P. Bruneval, A. A. Hagege, and P. Menasche
Enhancement of the functional benefits of skeletal myoblast transplantation by means of coadministration of hypoxia-inducible factor 1{alpha}
J. Thorac. Cardiovasc. Surg., July 1, 2005; 130(1): 173 - 179.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
T. Kofidis, J. L. de Bruin, T. Yamane, M. Tanaka, D. R. Lebl, R.-J. Swijnenburg, I. L. Weissman, and R. C. Robbins
Stimulation of Paracrine Pathways With Growth Factors Enhances Embryonic Stem Cell Engraftment and Host-Specific Differentiation in the Heart After Ischemic Myocardial Injury
Circulation, May 17, 2005; 111(19): 2486 - 2493.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
G. Keller
Embryonic stem cell differentiation: emergence of a new era in biology and medicine
Genes & Dev., May 15, 2005; 19(10): 1129 - 1155.
[Abstract] [Full Text] [PDF]

Home page
 J CARDIOVASC PHARMACOL THERHome page
A. Schuh, S. Breuer, R. Al Dashti, N. Sulemanjee, P. Hanrath, C. Weber, B. F. Uretsky, and E. R. Schwarz
Administration of Vascular Endothelial Growth Factor Adjunctive to Fetal Cardiomyocyte Transplantation and Improvement of Cardiac Function in the Rat Model
Journal of Cardiovascular Pharmacology and Therapeutics, January 1, 2005; 10(1): 55 - 66.
[Abstract] [PDF]

Home page
 Stem CellsHome page
T. Kofidis, J. L. de Bruin, T. Yamane, L. B. Balsam, D. R. Lebl, R.-J. Swijnenburg, M. Tanaka, I. L. Weissman, and R. C. Robbins
Insulin-Like Growth Factor Promotes Engraftment, Differentiation, and Functional Improvement after Transfer of Embryonic Stem Cells for Myocardial Restoration
Stem Cells, December 1, 2004; 22(7): 1239 - 1245.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. M. Hodgson, A. Behfar, L. V. Zingman, G. C. Kane, C. Perez-Terzic, A. E. Alekseev, M. Puceat, and A. Terzic
Stable benefit of embryonic stem cell therapy in myocardial infarction
Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H471 - H479.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
M. A. Retuerto, P. Schalch, G. Patejunas, J. Carbray, N. Liu, K. Esser, R. G. Crystal, and T. K. Rosengart
Angiogenic pretreatment improves the efficacy of cellular cardiomyoplasty performed with fetal cardiomyocyte implantation
J. Thorac. Cardiovasc. Surg., April 1, 2004; 127(4): 1041 - 1050.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
Y.-F. Xiao, J.-Y. Min, and J. P. Morgan
Immunosuppression and xenotransplantation of cells for cardiac repair
Ann. Thorac. Surg., February 1, 2004; 77(2): 737 - 744.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
Y. CHEN, Q. KE, Y. YANG, J. S. RANA, J. TANG, J. P. MORGAN, and Y.-F. XIAO
Cardiomyocytes overexpressing TNF-{alpha} attract migration of embryonic stem cells via activation of p38 and c-Jun amino-terminal kinase
FASEB J, December 1, 2003; 17(15): 2231 - 2239.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
C. Weikert, M. Eppenberger-Eberhardt, and H. M Eppenberger
Cellular engineering of ventricular adult rat cardiomyocytes
Cardiovasc Res, October 1, 2003; 59(4): 874 - 882.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Irie, I. B. Krukenkamp, J. F. F. Brinkmann, G. R. Gaudette, A. E. Saltman, W. Jou, J. F. C. Glatz, N. A. Abumrad, and A. Ibrahimi
Myocardial recovery from ischemia is impaired in CD36-null mice and restored by myocyte CD36 expression or medium-chain fatty acids
PNAS, May 27, 2003; 100(11): 6819 - 6824.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. G. Nir, R. David, M. Zaruba, W.-M. Franz, and J. Itskovitz-Eldor
Human embryonic stem cells for cardiovascular repair
Cardiovasc Res, May 1, 2003; 58(2): 313 - 323.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
J. D. Dowell, M. Rubart, K. B.S. Pasumarthi, M. H. Soonpaa, and L. J. Field
Myocyte and myogenic stem cell transplantation in the heart
Cardiovasc Res, May 1, 2003; 58(2): 336 - 350.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
A. Luttun and P. Carmeliet
De novo vasculogenesis in the heart
Cardiovasc Res, May 1, 2003; 58(2): 378 - 389.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
Q. He, J. Li, E. Bettiol, and M. E. Jaconi
Embryonic Stem Cells: New Possible Therapy for Degenerative Diseases That Affect Elderly People
J. Gerontol. A Biol. Sci. Med. Sci., March 1, 2003; 58(3): M279 - 287.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online