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J Appl Physiol 92: 2608-2616, 2002; doi:10.1152/japplphysiol.01067.2001
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Vol. 92, Issue 6, 2608-2616, June 2002

Experimental and calculated parameters on particle phagocytosis by alveolar macrophages

Per Camner1, Margot Lundborg1, Lena Låstbom1, Per Gerde1, Norma Gross1,2,3, and Connie Jarstrand2

1 Division of Inhalation Toxicology, Institute of Environmental Medicine, Karolinska Institutet, SE-171 77 Stockholm; 2 Division of Clinical and Oral Bacteriology, Department of Immunology, Microbiology, Pathology and Infectious Diseases, Huddinge University Hospital, SE-141 86 Huddinge, Sweden; and 3 Faculty of Microbiology, University of Costa Rica, San Juan, Costa Rica

Phagocytosis of three types of fluorescein-labeled test particles by rat alveolar macrophages (AM) were studied: spherical silica (3.2 µm), heat-killed Candida albicans (3.8 µm), and heat-killed Cryptococcus neoformans (6.1 µm) opsonized with specific IgG. These particles should attach to scavenger, mannose, and Fc receptors, respectively. Both control AM and AM pretreated for 20 h with interferon-gamma (12.5 or 50 U/ml) were studied. The sum of the number of attached and ingested particles per AM (accumulated attachment) was used as a measure of the attachment process, and the number of ingested particles per AM divided by the accumulated attachment (ingested fraction) was used as a measure of the ingestion process. The average ingestion time (IT), which is also a measure of the ingestion process, was calculated from the experimental data. The ingestion process was independent of the attachment process. IT increased with the time of observation. This is explained by the fact that IT determined from observation times shorter than the whole distribution of IT for a certain particle results in a shorter IT than the real average IT. C. albicans (mannose receptor) had the fastest ingestion process, C. neoformans opsonized with specific IgG (Fc receptor) had ingestion that was nearly as fast, and the silica particles (scavenger receptors) had the slowest ingestion process. Treatment with interferon-gamma markedly impaired the attachment process for all three types of particles (and three types of receptors) but clearly impaired the ingestion process only for silica particles (scavenger receptors).

macrophage receptors; interferon-gamma ; Candida albicans; Cryptococcus neoformans


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