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J Appl Physiol 92: 2264-2272, 2002; doi:10.1152/japplphysiol.01174.2001
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Vol. 92, Issue 6, 2264-2272, June 2002

Content of intramyocellular lipids derived by electron microscopy, biochemical assays, and 1H-MR spectroscopy

Hans Howald1, Chris Boesch2, Roland Kreis2, Sibylle Matter1, Rudolf Billeter1, Birgitta Essen-Gustavsson3, and Hans Hoppeler1

Departments of 1 Anatomy and 2 Clinical Research (Magnetic Resonance Spectroscopy and Methodology), University of Bern, CH-3000 Bern, Switzerland; and 3 Unit of Comparative Medicine and Physiology, Department of Large Animal Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, S-750 07 Uppsala, Sweden

Three different methods to determine intramyocellular lipid (IMCL) contents in human skeletal muscle have been compared. 1H-magnetic resonance spectroscopy (MRS) was evaluated against electron microscopic morphometry and biochemical assays of biopsy samples from m. tibialis anterior of 10 healthy subjects. The results of 1H-MRS and morphometry were strongly correlated, proving the validity of the 1H-MRS results for the noninvasive determination of IMCL. Biochemical assays yielded results that did not significantly correlate with the results of the other methods. When IMCL levels obtained from the three methods are expressed in common units, it was found that 1H-MRS yielded IMCL average levels that were 1.8 times lower than those found by morphometry. Potential reasons for the discrepancy are discussed. It is expected that 1H-MRS will be suitable to replace invasive techniques for IMCL determination, whenever noninvasiveness is crucial, e.g., for repeated investigations in studies of substrate recruitment and recovery in exercise.

skeletal muscle; exercise; energy substrates; quantitation; magnetic resonance


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