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J Appl Physiol 92: 2169-2176, 2002. First published January 11, 2002; doi:10.1152/japplphysiol.00927.2001
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Vol. 92, Issue 5, 2169-2176, May 2002

Characterization of LPS-induced lung inflammation in cftrminus /minus mice and the effect of docosahexaenoic acid

Steven D. Freedman1, Deborah Weinstein1, Paola G. Blanco1, Pedro Martinez-Clark1, Serge Urman1, Munir Zaman1, Jason D. Morrow2, and Juan G. Alvarez3

Departments of 1 Medicine and 3 Obstetrics and Gynecology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215; and 2 Departments of Medicine and Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

The mechanism by which Pseudomonas causes excessive inflammation in the cystic fibrosis lung is unclear. We have reported that arachidonic acid is increased and docosahexaenoic acid (DHA) decreased in lung, pancreas, and ileum from cftr-/- mice. Oral DHA corrected this defect and reversed the pathology. To determine which mediators regulate inflammation in lungs from cftr-/- mice and whether inhibition occurs with DHA, cftr-/- and wild-type (WT) mice were exposed to aerosolized Pseudomonas lipopolysaccharide (LPS). After 2 days of LPS, tumor necrosis factor-alpha (TNF-alpha ), macrophage inflammatory protein-2, and KC levels in bronchoalveolar lavage fluid were increased in cftr-/- compared with WT mice and not suppressed by pretreatment with oral DHA. Neutrophil levels were not different between cftr-/- and WT mice. After 3 days of aerosolized LPS, neutrophil concentration, TNF-alpha , and the eicosanoids 6-keto-PGF1alpha , PGF2alpha , PGE2, and thromboxane B2 were all increased in bronchoalveolar lavage fluid from cftr-/- mice compared with WT controls. Oral DHA had no significant effect on TNF-alpha levels in cftr-/- mice. In contrast, neutrophils and eicosanoids were decreased in cftr-/- but not in WT mice treated with DHA, indicating that the effects of DHA on these inflammatory parameters may be related to correction of the membrane lipid defect.

cystic fibrosis; cytokines; neutrophils; Pseudomonas


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