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1 Division of Nuclear Medicine, Department of Radiology, and 3 Surgical Service, Massachusetts General Hospital, and 2 Shriners Burns Institute, Boston 02114; and Departments of 4 Radiology and 5 Surgery, Harvard Medical School, Boston, Massachusetts 02115
Positron emission tomography
(PET) with H215O was used as an in vivo,
relatively noninvasive, quantitative method for measuring regional blood flow to hindlimb skeletal muscle of anesthetized dogs. A hydrooccluder positioned on the femoral artery was used to reduce flow, and high-flow states were produced by local infusion of
adenosine. Three to four measurements were made in each animal. Approximately 40 mCi of H215O were injected
intravenously, and serial images and arterial blood samples were
acquired over 2.5 min. Data analysis was performed by fitting tissue
and arterial blood time-activity curves to a modified,
single-compartment Kety model. The model equation was also solved on a
pixel-by-pixel basis to yield maps of regional skeletal muscle blood
flow. After each PET determination, flow was measured with
radioactive microspheres. Results of the PET measurements demonstrated
that basal flow to hindlimb skeletal muscle was 3.83 ± 0.36 ml · min
1 · 100 g1
(mean ± SE). This value was in excellent agreement with the
microsphere data, 3.73 ± 0.32 ml · min1 · 100 g1
(P = 0.69, not significant). Adenosine infusion
resulted in flows as high as 30 ml · min1 · 100 g1, and the
PET and microsphere data were highly correlated over the entire range
of flows (r2 = 0.98, P < 0.0001). We conclude that muscle blood flow can be accurately measured
in vivo by PET with H215O and that this
approach offers promise for application in human studies of muscle
metabolism under varying pathophysiological states.
hindlimb; microspheres; Doppler flow probe; dogs
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