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J Appl Physiol 92: 1443-1450, 2002. First published December 14, 2001; doi:10.1152/japplphysiol.00782.2001
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Vol. 92, Issue 4, 1443-1450, April 2002

Effect of the cytoskeletal prestress on the mechanical impedance of cultured airway smooth muscle cells

Dimitrije Stamenovic'1, Zhuangli Liang1, Jianxin Chen2, and Ning Wang2

1 Department of Biomedical Engineering, Boston University, Boston 02215; and 2 Physiology Program, Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115

We investigated the effect of the cytoskeletal prestress (P) on the elastic and frictional properties of cultured human airway smooth muscle cells during oscillatory loading; P is preexisting tensile stress in the actin cytoskeleton generated by the cell contractile apparatus. We oscillated (0.1 Hz, 6 Pa peak to peak) small ferromagnetic beads bound to integrin receptors and computed the storage (elastic) modulus (G') and the loss (frictional) modulus (G") from the applied torque and the corresponding bead rotation. All measurements were done at baseline and after cells were treated with graded doses of either histamine (0.1, 1, 10 µM) or isoproterenol (0.01, 0.1, 1, 10 µM). Values for P for these concentrations were taken from a previous study (Wang et al., Am J Physiol Cell Physiol, in press). It was found that G' and G", as well as P, increased/decreased with increasing doses of histamine/isoproterenol. Both G' and G" exhibited linear dependences on P: G'(Pa) = 0.20P + 82 and G"(Pa) = 0.05P + 32. The dependence of G' on P is consistent with our previous findings and with the behavior of stress-supported structures. The dependence of G" on P is a novel finding. It could be attributed to a variety of mechanisms. Some of those mechanisms are discussed in detail. We concluded that, in addition to the central mechanisms by which stress-supported structures develop mechanical stresses, other mechanisms might need to be invoked to fully explain the observed dependence of the cell mechanical properties on the state of cell contractility.

storage modulus; loss modulus; oscillatory cytometry; actin network; cytoskeletal mechanics


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