Vol. 92, Issue 2, 535-540, February 2002
Time course of changes in lipoprotein lipase activity in rat
skeletal muscles during denervation-reinnervation
E.
ernicka1,
E.
Smol2,
J.
Langfort1, and
M.
Górecka1
1 Department of Applied Physiology, Medical Research Center,
02-106 Warsaw; and 2 Department of Physiology, Academy of
Physical Education, 40-065 Katowice, Poland
The effects of
denervation-reinnervation after sciatic nerve crush on the activity of
extracellular and intracellular lipoprotein lipase (LPL) were examined
in the soleus and red portion of gastrocnemius muscles. The activity of
both LPL fractions was decreased in the two muscles within 24 h
after the nerve crush and remained reduced for up to 2 wk. During the
reinnervation period, LPL activity was still reduced in the soleus and
started to increase only on the 40th day. In the red gastrocnemius, LPL
activity increased progressively with reinnervation, exceeding control
values on the 30th day postcrush. The LPL activity in the soleus from
the contralateral to denervated hindlimb was also affected, being increased on the postoperation day and then gradually decreased during
the following days. In conclusion, the time course of changes in muscle LPL activity after nerve crush confirmed the predominant role
of nerve conduction in controlling muscle potential to take up free
fatty acids derived from the plasma triacylglycerols. However, other
factors, such as muscle fiber composition and the fiber transformation,
should also be considered in this aspect of the
denervation-reinnervation process. Moreover, it was found that
denervation of muscles from one hindlimb may influence LPL activity in
muscles from the contralateral leg.
skeletal muscle; denervation; reinnervation
