Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 92: 461-468, 2002; doi:10.1152/japplphysiol.01152.2000
8750-7587/02 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by King-VanVlack, C. E.
Right arrow Articles by MacDonald, P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by King-VanVlack, C. E.
Right arrow Articles by MacDonald, P. H.
Vol. 92, Issue 2, 461-468, February 2002

Endothelial modulation of skeletal muscle blood flow and VO2 during low- and high-intensity contractions

Cheryl E. King-VanVlack1,2, J. D. Mewburn2, C. K. Chapler2, and P. H. MacDonald3

1 School of Rehabilitation Therapy, 2 Department of Physiology, and 3 Department of Surgery, Queen's University, Kingston, Ontario, Canada K7L 3N6

In the present study, we determined whether endothelin (ET)-1 contributed to the observed reduction in muscle blood flow (Q) during contractions with nitric oxide synthase (NOS) inhibition and whether muscle O2 uptake (VO2) would be affected by the decrease in muscle Q with NOS inhibition at different contraction intensities. Muscle Q, VO2, O2 extraction ratio (OER), and tension development (TD) were studied in the in situ gastrocnemius muscle preparation in anesthetized dogs. A decrease in the VO2-to-TD ratio (VO2/TD) was used as an indicator of O2 limitation. Three contraction protocols were used: 1) isometric twitch contractions at 2 twitches (tw)/s, 2) the same contractions at 4 tw/s, and 3) pretreatment with an ETA-receptor antagonist (BQ-123) before 2 tw/s contractions. The muscle was stimulated to contract, and measures were obtained at steady state (~5-8 min). NOS inhibition (Nomega -nitro-L-arginine methyl ester) was then induced, and measures were repeated at 2, 5, 10, and 15 min. During 2 tw/s contractions, NOS inhibition reduced Q with and without ETA-receptor blockade. In both groups, OER increased in response to the fall in Q, with the result being no change in VO2/TD. NOS inhibition also decreased Q during 4 tw/s contractions, but OER did not increase, resulting in a reduction in VO2/TD 5 and 15 min after Nomega -nitro-L-arginine methyl ester. These data indicated that 1) a reciprocal increase in ET-1 during NOS inhibition does not influence active hyperemia in skeletal muscle, and 2) during 4 tw/s contractions, the ischemia with NOS inhibition was associated with either an O2 limitation or an alteration in the efficiency of muscle contractions.

fatigue; oxygen extraction; tension development; oxygen demand


This article has been cited by other articles:


Home page
 J Am Coll CardiolHome page
M. Guazzi, M. Samaja, R. Arena, M. Vicenzi, and M. D. Guazzi
Long-Term Use of Sildenafil in the Therapeutic Management of Heart Failure
J. Am. Coll. Cardiol., November 27, 2007; 50(22): 2136 - 2144.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. P. Mortensen, J. Gonzalez-Alonso, R. Damsgaard, B. Saltin, and Y. Hellsten
Inhibition of nitric oxide and prostaglandins, but not endothelial-derived hyperpolarizing factors, reduces blood flow and aerobic energy turnover in the exercising human leg
J. Physiol., June 1, 2007; 581(2): 853 - 861.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Guazzi, M. Berti, S. Belletti, G. Reina, and M. D. Guazzi
Exercise metaboreflex activation and endothelial function impairment in atrial fibrillation
Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2396 - H2402.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Guazzi, S. Belletti, E. Bianco, L. Lenatti, and M. D. Guazzi
Endothelial dysfunction and exercise performance in lone atrial fibrillation or associated with hypertension or diabetes: different results with cardioversion
Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H921 - H928.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. Shibata, K. Qin, S. Ichioka, and A. Kamiya
Vascular wall energetics in arterioles during nitric oxide-dependent and -independent vasodilation
J Appl Physiol, June 1, 2006; 100(6): 1793 - 1798.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
D. J. Baker, D. J. Krause, R. A. Howlett, and R. T. Hepple
Nitric oxide synthase inhibition reduces O2 cost of force development and spares high-energy phosphates following contractions in pump-perfused rat hindlimb muscles
Exp Physiol, May 1, 2006; 91(3): 581 - 589.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Shibata, S. Ichioka, and A. Kamiya
Nitric oxide modulates oxygen consumption by arteriolar walls in rat skeletal muscle
Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2673 - H2679.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
B. Grassi, M. C Hogan, K. M Kelley, R. A Howlett, and L. B. Gladden
Effects of nitric oxide synthase inhibition by L-NAME on oxygen uptake kinetics in isolated canine muscle in situ
J. Physiol., November 1, 2005; 568(3): 1021 - 1033.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
D. J Krause, J. L Hagen, C. A Kindig, and R. T Hepple
Nitric oxide synthase inhibition reduces the O2 cost of force development in rat hindlimb muscles pump perfused at matched convective O2 delivery
Exp Physiol, November 1, 2005; 90(6): 889 - 900.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online