Journal of Applied Physiology
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J Appl Physiol 92: 288-296, 2002;
8750-7587/02 $5.00
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Vol. 92, Issue 1, 288-296, January 2002

Transplantation of embryonic stem cells improves cardiac function in postinfarcted rats

Jiang-Yong Min1, Yinke Yang1, Kimber L. Converso1, Lixin Liu1, Qin Huang2, James P. Morgan1, and Yong-Fu Xiao1

1 The Charles A. Dana Research Institute and the Harvard-Thorndike Laboratory; Cardiovascular Division, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston 02215; and 2 Department of Pathology, The Holy Family Hospital and Medical Center, Methuen, Massachusetts 01884

Massive loss of cardiac myocytes after myocardial infarction (MI) is a common cause of heart failure. The present study was designed to investigate the improvement of cardiac function in MI rats after embryonic stem (ES) cell transplantation. MI in rats was induced by ligation of the left anterior descending coronary artery. Cultured ES cells used for cell transplantation were transfected with the marker green fluorescent protein (GFP). Animals in the treated group received intramyocardial injection of ES cells in injured myocardium. Compared with the MI control group injected with an equivalent volume of the cell-free medium, cardiac function in ES cell-implanted MI animals was significantly improved 6 wk after cell transplantation. The characteristic phenotype of engrafted ES cells was identified in implanted myocardium by strong positive staining to sarcomeric alpha -actin, cardiac alpha -myosin heavy chain, and troponin I. GFP-positive cells in myocardium sectioned from MI hearts confirmed the survival and differentiation of engrafted cells. In addition, single cells isolated from cell-transplanted MI hearts showed rod-shaped GFP-positive myocytes with typical striations. The present data demonstrate that ES cell transplantation is a feasible and novel approach to improve ventricular function in infarcted failing hearts.

cell transplantation; myocardial infarction


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