Journal of Applied Physiology Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 91: 2233-2239, 2001;
8750-7587/01 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heunks, L. M. A.
Right arrow Articles by Sieck, G. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heunks, L. M. A.
Right arrow Articles by Sieck, G. C.
Vol. 91, Issue 5, 2233-2239, November 2001

Nitric oxide impairs Ca2+ activation and slows cross-bridge cycling kinetics in skeletal muscle

Leo M. A. Heunks1, Mark J. Cody2, Paige C. Geiger2, P. N. Richard Dekhuijzen1, and Gary C. Sieck2

1 Department of Pulmonary Diseases, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands; and 2 Departments of Anesthesiology and Physiology and Biophysics, Mayo Clinic and Foundation, Rochester, Minnesota 55902

The effects of the nitric oxide (NO) donor spermine NONOate (Sp-NO, 1.0 mM) on cross-bridge recruitment and cross-bridge cycling kinetics were studied in permeabilized rabbit psoas muscle fibers. Fibers were activated at various Ca2+ concentrations (pCa, negative logarithm of Ca2+ concentration), and the pCa at which force was maximal (pCa 4.0) and ~50% of maximal (pCa50 5.6) were determined. Fiber stiffness was determined using 1-kHz sinusoidal length perturbations, and the fraction of cross bridges in the force-generating state was estimated by the ratio of stiffness during maximal (pCa 4.0) and submaximal (pCa 5.6) Ca2+ activation to stiffness during rigor (at pCa 4.0). Cross-bridge cycling kinetics were evaluated by measuring the rate constant for force redevelopment after quick release (by 15% of optimal fiber length, Lo) and restretch of the fiber to Lo. Exposing fibers to Sp-NO for 10 min reduced force and the fraction of cross bridges in the force-generating state at maximal and submaximal (pCa50) Ca2+ activation. However, the effects of Sp-NO were more pronounced during submaximal Ca2+ activation. Sp-NO also reduced the rate constant for force redevelopment but only during submaximal Ca2+ activation. We conclude that Sp-NO reduces Ca2+ sensitivity by decreasing the number of cross bridges in the strongly bound state and also impairs cross-bridge cycling kinetics during submaximal activation.

rabbit psoas; spermine NONOate; rate of force redevelopment; stiffness


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. W. H. van Hees, Y.-P. Li, C. A. C. Ottenheijm, B. Jin, C. J. C. Pigmans, M. Linkels, P. N. R. Dekhuijzen, and L. M. A. Heunks
Proteasome inhibition improves diaphragm function in congestive heart failure rats
Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1260 - L1268.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
E. Prochniewicz, D. A. Lowe, D. J. Spakowicz, L. Higgins, K. O'Conor, L. V. Thompson, D. A. Ferrington, and D. D. Thomas
Functional, structural, and chemical changes in myosin associated with hydrogen peroxide treatment of skeletal muscle fibers
Am J Physiol Cell Physiol, February 1, 2008; 294(2): C613 - C626.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
D. J. Baker, D. J. Krause, R. A. Howlett, and R. T. Hepple
Nitric oxide synthase inhibition reduces O2 cost of force development and spares high-energy phosphates following contractions in pump-perfused rat hindlimb muscles
Exp Physiol, May 1, 2006; 91(3): 581 - 589.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. A. C. Ottenheijm, L. M. A. Heunks, M. C. P. Geraedts, and P. N. R. Dekhuijzen
Hypoxia-induced skeletal muscle fiber dysfunction: role for reactive nitrogen species
Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L127 - L135.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. A. C. Ottenheijm, L. M. A. Heunks, G. C. Sieck, W.-Z. Zhan, S. M. Jansen, H. Degens, T. de Boo, and P. N. R. Dekhuijzen
Diaphragm Dysfunction in Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med., July 15, 2005; 172(2): 200 - 205.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. T. Corr and W. Herzog
Force recovery after activated shortening in whole skeletal muscle: transient and steady-state aspects of force depression
J Appl Physiol, July 1, 2005; 99(1): 252 - 260.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. M Jones, D. P Wilkerson, and I. T Campbell
Nitric oxide synthase inhibition with L-NAME reduces maximal oxygen uptake but not gas exchange threshold during incremental cycle exercise in man
J. Physiol., October 1, 2004; 560(1): 329 - 338.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
X. Zhu, L. M. A. Heunks, H. A. Machiels, L. Ennen, and P. N. R. Dekhuijzen
Effects of modulation of nitric oxide on rat diaphragm isotonic contractility during hypoxia
J Appl Physiol, February 1, 2003; 94(2): 612 - 620.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online