Journal of Applied Physiology Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 91: 2102-2108, 2001;
8750-7587/01 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Paulsen, S. R.
Right arrow Articles by Winder, W. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paulsen, S. R.
Right arrow Articles by Winder, W. W.
Vol. 91, Issue 5, 2102-2108, November 2001

AMP-activated protein kinase activation prevents denervation-induced decline in gastrocnemius GLUT-4

S. R. Paulsen, D. S. Rubink, and W. W. Winder

Department of Zoology, Brigham Young University, Provo, Utah 84602

This study was designed to determine whether the reductions in GLUT-4 seen in 3-day-denervated muscles can be prevented through chemical activation of 5'-AMP-activated protein kinase (AMPK). Muscle AMPK can be chemically activated in rats using subcutaneous injections with 5-aminoimidazole-4-carboxamide-1-beta -D-ribofuranoside (AICAR). In this study, the tibial nerve was sectioned on one side; the other was sham operated but without nerve section. Acute injections of AICAR resulted in significantly increased AMPK activity in denervated gastrocnemius but not soleus muscles. Acetyl-CoA carboxylase activity, a reporter of AMPK activation, declined in both gastrocnemius and soleus in both denervated and contralateral muscles. Three days after denervation, GLUT-4 levels were significantly decreased by ~40% in gastrocnemius muscles and by ~30% in soleus muscles. When rats were injected with AICAR (1 mg/g body wt) for 3 days, the decline in GLUT-4 levels was prevented in denervated gastrocnemius muscles but not in denervated soleus muscles. The extent of denervation-induced muscle atrophy was similar in AICAR-treated vs. saline-treated rats. These studies provide evidence that some effects of denervation may be prevented by chemical activation of the appropriate signaling pathways.

5-aminoimidazole-4-carboxamide-1-beta -D-ribofuranoside; adenosine 5'-monophosphate; acetyl-coenzyme A carboxylase; glucose transport; contraction


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. L. G. S. Oliveira, M. Ueno, C. T. de Souza, M. Pereira-da-Silva, A. L. Gasparetti, R. M. N. Bezzera, L. C. Alberici, A. E. Vercesi, M. J. A. Saad, and L. A. Velloso
Cold-induced PGC-1{alpha} expression modulates muscle glucose uptake through an insulin receptor/Akt-independent, AMPK-dependent pathway
Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E686 - E695.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
B. F. Holmes, D. B. Lang, M. J. Birnbaum, J. Mu, and G. L. Dohm
AMP kinase is not required for the GLUT4 response to exercise and denervation in skeletal muscle
Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E739 - E743.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. P. Y. Koonen, C. R. Benton, Y. Arumugam, N. N. Tandon, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen
Different mechanisms can alter fatty acid transport when muscle contractile activity is chronically altered
Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E1042 - E1049.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. L. Dohm
Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Regulation of skeletal muscle GLUT-4 expression by exercise
J Appl Physiol, August 1, 2002; 93(2): 782 - 787.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
P. E. Durante, K. J. Mustard, S.-H. Park, W. W. Winder, and D. G. Hardie
Effects of endurance training on activity and expression of AMP-activated protein kinase isoforms in rat muscles
Am J Physiol Endocrinol Metab, July 1, 2002; 283(1): E178 - E186.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online