Journal of Applied Physiology Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 91: 1941-1947, 2001;
8750-7587/01 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fernandez, R.
Right arrow Articles by Younger, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fernandez, R.
Right arrow Articles by Younger, J. G.
Vol. 91, Issue 5, 1941-1947, November 2001

Exposure to perflubron is associated with decreased Syk phosphorylation in human neutrophils

Rosemarie Fernandez1, Vidya Sarma2, Ellen Younkin2, Ronald B. Hirschl3, Peter A. Ward2, and John G. Younger1

Departments of 1 Emergency Medicine, 2 Pathology, and 3 Surgery, University of Michigan, Ann Arbor, Michigan 48109-0303

Liquid ventilation with perflubron is associated with reduced neutrophil recruitment into the lung during acute injury. Perflubron also reduces chemotactic responses, the respiratory burst, and cytokine production in neutrophils and in alveolar macrophages in vitro. In the current studies, the effect of perflubron on neutrophil chemotaxis to formyl-Met-Leu-Phe (fMLP) and phagocytosis of opsonized sheep erythrocytes (EA) correlated with decreased phosphorylation of Syk, an important intracellular second messenger in pathways regulating neutrophil functional responses. Brief (5 min) exposure of neutrophils to perflubron resulted in a dose-dependent reduction in chemotaxis to fMLP and reduced phagocytosis of EA but no apparent morphological changes as seen by electron microscopy. Concurrently, there was a reduction in both total cytosolic tyrosine phosphorylation and Syk phosphorylation. Binding studies indicated that this effect was neither a result of impaired ligand-receptor affinity nor a change in the number of fMLP receptors available on the neutrophil surface. These results suggest that perflubron nonspecifically affects cellular activation as measured by tyrosine phosphorylation perhaps by interfering with transmembrane signal transduction.

partial liquid ventilation; perfluorocarbon; acute lung injury; tyrosine kinase; Fcgamma RIIA


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Haufe, E. Koenigshausen, L. Knels, M. Wendel, S. N. Stehr, and T. Koch
Leukocyte antibacterial functions are not impaired by perfluorocarbon exposure in vitro
Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L134 - L142.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. Burkhardt, P. Koehne, H. Wissel, S. Graf, H. Proquitte, R. R. Wauer, and M. Rudiger
Intratracheal perfluorocarbons diminish LPS-induced increase in systemic TNF-{alpha}
Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1043 - L1048.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Haufe, T. Luther, M. Kotzsch, L. Knels, and T. Koch
Perfluorocarbon attenuates response of concanavalin A-stimulated mononuclear blood cells without altering ligand-receptor interaction
Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L210 - L216.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. von der Hardt, M. A. Kandler, L. Fink, E. Schoof, J. Dotsch, R. M. Bohle, and W. Rascher
Laser-assisted microdissection and real-time PCR detect anti-inflammatory effect of perfluorocarbon
Am J Physiol Lung Cell Mol Physiol, July 1, 2003; 285(1): L55 - L62.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
H. A. Haeberle, F. Nesti, H.-J. Dieterich, Z. Gatalica, and R. P. Garofalo
Perflubron Reduces Lung Inflammation in Respiratory Syncytial Virus Infection by Inhibiting Chemokine Expression and Nuclear Factor-{kappa}B Activation
Am. J. Respir. Crit. Care Med., May 15, 2002; 165(10): 1433 - 1438.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online