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J Appl Physiol 91: 1814-1818, 2001;
8750-7587/01 $5.00
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Vol. 91, Issue 4, 1814-1818, October 2001

Decreased non-MHC-restricted (CD56+) killer cell cytotoxicity after spaceflight

Satish K. Mehta1, Indreshpal Kaur1, Elizabeth A. Grimm2, Christine Smid2, Daniel L. Feeback3, and Duane L. Pierson3

1 Enterprise Advisory Services Inc., Houston 77058; 2 Department of Tumor Biology, The University of Texas M. D. Anderson Cancer Center, Houston 77030; and 3 Life Sciences Research Laboratories, National Aeronautics and Space Administration, Johnson Space Center, Houston, Texas 77058

Cytotoxic activity of non-major histocompatibility complex-restricted (CD56+) (NMHC) killer cells and cell surface marker expression of peripheral blood mononuclear cells were determined before and after spaceflight. Ten astronauts (9 men, 1 woman) from two space shuttle missions (9- and 10-day duration) participated in the study. Blood samples were collected 10 days before launch, within 3 h after landing, and 3 days after landing. All peripheral blood mononuclear cell preparations were cryopreserved and analyzed simultaneously in a 4-h cytotoxicity 51Cr release assay using K562 target cells. NMHC killer cell lytic activity was normalized per 1,000 CD56+ cells. When all 10 subjects were considered as one study group, NMHC killer cell numbers did not change significantly during the three sampling periods, but at landing lytic activity had decreased by ~40% (P < 0.05) from preflight values. Nine of ten astronauts had decreased lytic activity immediately after flight. NMHC killer cell cytotoxicity of only three astronauts returned toward preflight values by 3 days after landing. Consistent with decreased NMHC killer cell cytotoxicity, urinary cortisol significantly increased after landing compared with preflight levels. Plasma cortisol and ACTH levels at landing were not significantly different from preflight values. No correlation of changes in NMHC killer cell function or hormone levels with factors such as age, gender, mission, or spaceflight experience was found. After landing, expression of the major lymphocyte surface markers (CD3, CD4, CD8, CD14, CD16, CD56), as determined by flow cytometric analysis, did not show any consistent changes from measurements made before flight.

space immunology; microgravity; non-major histocompatibility complex-restricted killer cell


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