Journal of Applied Physiology AJP: Lung Cellular and Molecular Physiology
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J Appl Physiol 91: 1401-1411, 2001;
8750-7587/01 $5.00
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Vol. 91, Issue 3, 1401-1411, September 2001

Regulation of vocal fold transepithelial water fluxes

Kimberly V. Fisher1, Alvin Telser2, Jonathan E. Phillips3, and Donovan B. Yeates3

1 Department of Communication Sciences and Disorders, Northwestern University, Evanston 60208; 2 Department of Cell and Molecular Biology, Northwestern University, Chicago 60611; and 3 Department of Medicine, University of Illinois at Chicago, and Veterans Affairs Chicago Health Care System, Chicago, Illinois 60612

Vocal fold hydration is critical to phonation. We hypothesized that the vocal fold generates bidirectional water fluxes, which are regulated by activity of the Na+-K+- ATPase. Western blots and immunohistochemistry demonstrated the presence of the alpha -subunit Na+-K+-ATPase in the canine vocal fold (n = 11). Luminal cells, basal and adjacent one to two layers of suprabasal cells within stratified squamous epithelium, were immunopositive, as well as basolateral membranes of submucosal seromucous glands underlying transitional epithelia. Canine (n = 6) and ovine (n = 14) vocal fold mucosae exhibited transepithelial potential differences of 8.1 ± 2.8 and 9.3 ± 1.3 mV (lumen negative), respectively. The potential difference and short-circuit current (ovine = 31 ± 4 µA/cm2; canine = 41 ± 10 µA/cm2) were substantially reduced by luminal administration of 75 µM acetylstrophanthidin (P < 0.05). Ovine (n = 7) transepithelial water fluxes decreased from 5.1 ± 0.3 to 4.3 ± 0.3 µl · min-1 · cm-2 from the basal to luminal chamber and from 5.2 ± 0.2 to 3.9 ± 0.3 µl · min-1 · cm-2 from the luminal to basal chamber by luminal acetylstrophanthidin (P < 0.05). The presence of the Na+-K+-ATPase in the vocal fold epithelium and the electrolyte transport derived from its activity provide the intrinsic mechanisms to regulate cell volume as well as vocal fold hydration.

ion transport; sodium-potassium pump; larynx; voice; dog


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