|
|
||||||||
Department of Pharmacology, Midwestern University, Downers Grove, Illinois 60515
The inherent electrical
resistance of the rat vaginal wall rises markedly near the beginning of
estrus and then falls again to low levels for the remainder of the
ovarian cycle. Accordingly, special instruments have been developed to
measure such resistances (within seconds) on simply inserting a small
probe fitted with a pair of recording electrodes into the vagina (i.e.,
the MK-10A impedance checker and the EC40 estrus cycle monitor). As
described herein, these two instruments are far more convenient for
monitoring individual cycles than more laborious methods in which
vaginal smears are inspected for changes in numbers of cornified (C), nucleated (N), and leukocytic (L) cells. However, they are also expensive and their use has essentially remained uncited in the literature. Thus we sought to determine whether a simple, inexpensive electrical meter (with resistance-measuring capacity), as commonly used
by professional electricians, would serve the same purpose. We chose a
standard multifunctional meter (model 22-178, RadioShack) and attached
leads to it fabricated from the internal wiring of a shielded
audiocable (model 42-2387A, RadioShack), one male terminal of which was
used as a vaginal probe. In rats from which vagina smears
revealed cell numbers in the order of C > N > L (typical of
early estrus) electrical resistances were high, 488 ± 130 k
(18 rats). In rats from which vagina smears revealed all other possible
cell distributions, electrical resistances (combined) were much lower
(P < 0.05), 124 ± 23 k
(32 rats). Thus
readily accessible, inexpensive electrical meters may be useful in
assessing the status of estrus in female rats, either to improve
reproductive efficiencies and/or for other purposes involving
experiments in which such information is desirable.
estrus; female rat; tissue
This article has been cited by other articles:
![]() |
N. M. Thompson, A. M. Norman, S. S. Donkin, R. R. Shankar, M. H. Vickers, J. L. Miles, and B. H. Breier Prenatal and Postnatal Pathways to Obesity: Different Underlying Mechanisms, Different Metabolic Outcomes Endocrinology, May 1, 2007; 148(5): 2345 - 2354. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. M. Khan, M. Hadman, C. Wakade, L. M. De Sevilla, K. M. Dhandapani, V. B. Mahesh, R. K. Vadlamudi, and D. W. Brann Cloning, Expression, and Localization of MNAR/PELP1 in Rodent Brain: Colocalization in Estrogen Receptor-{alpha}- But Not in Gonadotropin-Releasing Hormone-Positive Neurons Endocrinology, December 1, 2005; 146(12): 5215 - 5227. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |