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Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan
To clarify the transport of O2 across the microvessels in skeletal muscle, we designed an intravital laser microscope that utilizes a phosphorescence quenching technique to determine both the microvascular and tissue PO2. After we injected the phosphorescent probe into systemic blood, phosphorescence excited by a N2-dye pulse laser was detected with a photomultiplier over a 10 µm in diameter area. In vitro and in vivo calibrations confirmed that the present method is accurate for PO2 measurements in the range of 7-90 Torr (r = 0.958) and has a rapid response time. This method was then used to measure the PO2 of microvessels with different diameters (40-130 µm) and of interstitial spaces in rat cremaster muscle. These measurements showed a significant drop in PO2 in the arterioles after branching (from 74.6 to 46.6 Torr) and the presence of a large PO2 gradient at the blood-tissue interface of arterioles (15-20 Torr). These findings suggest that capillaries are not the sole source of oxygen supply to surrounding tissue.
oxygen transport; intravital microscope; microcirculation; palladium-porphyrin; cremaster muscle
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