Journal of Applied Physiology AJP: Regulatory, Integrative and Comparative Physiology
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J Appl Physiol 90: 2460-2465, 2001;
8750-7587/01 $5.00
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Vol. 90, Issue 6, 2460-2465, June 2001

Relationship between myosin phosphorylation and contractile capability of canine airway smooth muscle

Richard W. Mitchell, Chun Y. Seow, Theodor Burdyga, Roberto Maass-Moreno, Victor R. Pratusevich, Joseph Ragozzino, and Lincoln E. Ford

Krannert Institute of Cardiology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46223

To better understand excitation-contraction coupling in smooth muscle, myosin phosphorylation and force-velocity properties of canine tracheal muscle were compared during the rise and early plateau of force in electrically stimulated tetani. Velocity reached a peak of ~1.5 times plateau value when force had risen to ~45% of its maximum value and then declined progressively. Except early in the tetanus, when phosphorylation rose rapidly, maximum power and phosphorylation had nearly parallel time courses, reaching peaks of 1.2-1.3 times reference at 6-8 s before declining to the plateau level at ~12 s. Force, velocity, maximum power, and phosphorylation fell somewhat during the plateau, with the closest correlation between phosphorylation and power. These results suggest that 1) early velocity slowing is not associated with light chain dephosphorylation and 2) maximum power, which we use to signal changes in activation, is closely correlated with the degree of light chain phosphorylation, at least when phosphorylation level is not changing rapidly. Dissociation of these two properties would be expected early in the tetanus if phosphorylation precedes mechanical activity.

tracheal smooth muscle; force-velocity relations; activation


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