| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Physiology and Biomechanics, National Institute of Fitness and Sports, Kanoya, Kagoshima 891-2393; 2 Division of Health Promotion and 3 Clinical Nutrition, National Institute of Health and Nutrition, Shinjuku Tokyo, 162-8636 Japan; and 4 Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110
This study was performed to assess the effects of short-term, extremely high-intensity intermittent exercise training on the GLUT-4 content of rat skeletal muscle. Three- to four-week-old male Sprague-Dawley rats with an initial body weight ranging from 45 to 55 g were used for this study. These rats were randomly assigned to an 8-day period of high-intensity intermittent exercise training (HIT), relatively high-intensity intermittent prolonged exercise training (RHT), or low-intensity prolonged exercise training (LIT). Age-matched sedentary rats were used as a control. In the HIT group, the rats repeated fourteen 20-s swimming bouts with a weight equivalent to 14, 15, and 16% of body weight for the first 2, the next 4, and the last 2 days, respectively. Between exercise bouts, a 10-s pause was allowed. RHT consisted of five 17-min swimming bouts with a 3-min rest between bouts. During the first bout, the rat swam without weight, whereas during the following four bouts, the rat was attached to a weight equivalent to 4 and 5% of its body weight for the first 5 days and the following 3 days, respectively. Rats in the LIT group swam 6 h/day for 8 days in two 3-h bouts separated by 45 min of rest. In the first experiment, the HIT, LIT, and control rats were compared. GLUT-4 content in the epitrochlearis muscle in the HIT and LIT groups after training was significantly higher than that in the control rats by 83 and 91%, respectively. Furthermore, glucose transport activity, stimulated maximally by both insulin (2 mU/ml) (HIT: 48%, LIT: 75%) and contractions (25 10-s tetani) (HIT: 55%, LIT: 69%), was higher in the training groups than in the control rats. However, no significant differences in GLUT-4 content or in maximal glucose transport activity in response to both insulin and contractions were observed between the two training groups. The second experiment demonstrated that GLUT-4 content after HIT did not differ from that after RHT (66% higher in trained rats than in control). In conclusion, the present investigation demonstrated that 8 days of HIT lasting only 280 s elevated both GLUT-4 content and maximal glucose transport activity in rat skeletal muscle to a level similar to that attained after LIT, which has been considered a tool to increase GLUT-4 content maximally.
epitrochlearis muscle; high-intensity exercise; intermittent training
This article has been cited by other articles:
|
|
 |
|
  K. A. Burgomaster, K. R. Howarth, S. M. Phillips, M. Rakobowchuk, M. J. MacDonald, S. L. McGee, and M. J. Gibala Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans J. Physiol., January 1, 2008; 586(1): 151 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hirasaka, S. Kohno, J. Goto, H. Furochi, K. Mawatari, N. Harada, T. Hosaka, Y. Nakaya, K. Ishidoh, T. Obata, et al. Deficiency of Cbl-b Gene Enhances Infiltration and Activation of Macrophages in Adipose Tissue and Causes Peripheral Insulin Resistance in Mice Diabetes, October 1, 2007; 56(10): 2511 - 2522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Burgomaster, N. M. Cermak, S. M. Phillips, C. R. Benton, A. Bonen, and M. J. Gibala Divergent response of metabolite transport proteins in human skeletal muscle after sprint interval training and detraining Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R1970 - R1976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Hohl, R. B. de Oliveira, D. Vaz de Macedo, and R. Brenzikofer Apparatus for measuring rat body volume: a methodological proposition J Appl Physiol, March 1, 2007; 102(3): 1229 - 1234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. H. Smith, M. Collins, L. A. Grobler, C. J. Magee, and E. O. Ojuka Exercise and CaMK activation both increase the binding of MEF2A to the Glut4 promoter in skeletal muscle in vivo Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E413 - E420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Gibala, J. P. Little, M. van Essen, G. P. Wilkin, K. A. Burgomaster, A. Safdar, S. Raha, and M. A. Tarnopolsky Short-term sprint interval versus traditional endurance training: similar initial adaptations in human skeletal muscle and exercise performance J. Physiol., September 15, 2006; 575(3): 901 - 911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Baar To perform your best: work hard not long J. Physiol., September 15, 2006; 575(3): 690 - 690. [Full Text] [PDF]
|
|
|
|
|
|
P. M. Garcia-Roves, J. Huss, and J. O. Holloszy Role of calcineurin in exercise-induced mitochondrial biogenesis Am J Physiol Endocrinol Metab, June 1, 2006; 290(6): E1172 - E1179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zoll, E. Ponsot, S. Dufour, S. Doutreleau, R. Ventura-Clapier, M. Vogt, H. Hoppeler, R. Richard, and M. Fluck Exercise training in normobaric hypoxia in endurance runners. III. Muscular adjustments of selected gene transcripts J Appl Physiol, April 1, 2006; 100(4): 1258 - 1266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Sigal, G. P. Kenny, D. H. Wasserman, and C. Castaneda-Sceppa Physical Activity/Exercise and Type 2 Diabetes Diabetes Care, October 1, 2004; 27(10): 2518 - 2539. [Full Text] [PDF]
|
|
|
|
|
|
S. Terada and I. Tabata Effects of acute bouts of running and swimming exercise on PGC-1{alpha} protein expression in rat epitrochlearis and soleus muscle Am J Physiol Endocrinol Metab, February 1, 2004; 286(2): E208 - E216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. O. OJUKA, T. E. JONES, D.-H. HAN, M. CHEN, and J. O. HOLLOSZY Raising Ca2+ in L6 myotubes mimics effects of exercise on mitochondrial biogenesis in muscle FASEB J, April 1, 2003; 17(6): 675 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Dohm Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Regulation of skeletal muscle GLUT-4 expression by exercise J Appl Physiol, August 1, 2002; 93(2): 782 - 787. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
