Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 90: 1532-1538, 2001;
8750-7587/01 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (24)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Olfert, I. M.
Right arrow Articles by Wagner, P. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Olfert, I. M.
Right arrow Articles by Wagner, P. D.
Vol. 90, Issue 4, 1532-1538, April 2001

Chronic hypoxia attenuates resting and exercise-induced VEGF, flt-1, and flk-1 mRNA levels in skeletal muscle

I. Mark Olfert1, Ellen C. Breen2, Odile Mathieu-Costello2, and Peter D. Wagner2

1 Department of Physiology and Pharmacology, Loma Linda University, Loma Linda 92350; and 2 Division of Physiology, Department of Medicine, University of California, San Diego, La Jolla, California 92093-0623

Vascular endothelial growth factor (VEGF) is a hypoxia-inducible angiogenic mitogen. However, chronic hypoxia is generally not found to increase mammalian skeletal muscle capillarity. We sought to determine the effect of chronic hypoxia (8 wk, inspired O2 fraction = 0.12) on skeletal muscle gene expression of VEGF, its receptors (flt-1 and flk-1), basic fibroblast growth factor, and transforming growth factor-beta 1. Wistar rats were exposed to chronic hypoxia (n = 12) or room air (n = 12). After the exposure period, six animals from each group were subjected to a single 1-h treadmill exercise bout (18 m/min on a 10° incline) in room air while the remaining six animals served as rest controls. Morphological analysis revealed that chronic hypoxia did not increase skeletal muscle capillarity. Northern blot analyses showed that chronic hypoxia decreased resting VEGF, flt-1, and flk-1 mRNA by 23, 68, and 42%, respectively (P < 0.05). The VEGF mRNA response to exercise was also decreased (4.1- and 2.7-fold increase in room air and chronic hypoxia, respectively, P < 0.05). In contrast, neither transforming growth factor-beta 1 nor basic fibroblast growth factor mRNA was significantly altered by chronic hypoxia. In conclusion, prolonged exposure to hypoxia attenuated gene expression of VEGF and its receptors flt-1 and flk-1 in rat gastrocnemius muscle. These findings may provide an explanation for the lack of mammalian skeletal muscle angiogenesis that is observed after chronic hypoxia.

Northern blot analysis; angiogenesis; transforming growth factor-beta 1; basic fibroblast growth factor


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
N. A. Ryan, K. A. Zwetsloot, L. M. Westerkamp, R. C. Hickner, W. E. Pofahl, and T. P. Gavin
Lower skeletal muscle capillarization and VEGF expression in aged vs. young men
J Appl Physiol, January 1, 2006; 100(1): 178 - 185.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
T. M. Yau, G. Li, Y. Zhang, R. D. Weisel, D. A.G. Mickle, and R.-K. Li
Vascular Endothelial Growth Factor Receptor Upregulation in Response to Cell-Based Angiogenic Gene Therapy
Ann. Thorac. Surg., June 1, 2005; 79(6): 2056 - 2063.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. E. Waters, S. Rotevatn, P. Li, B. H. Annex, and Z. Yan
Voluntary running induces fiber type-specific angiogenesis in mouse skeletal muscle
Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1342 - C1348.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
C. Lundby, H. Pilegaard, J. L. Andersen, G. van Hall, M. Sander, and J. A. L. Calbet
Acclimatization to 4100 m does not change capillary density or mRNA expression of potential angiogenesis regulatory factors in human skeletal muscle
J. Exp. Biol., October 15, 2004; 207(22): 3865 - 3871.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. M. Prior, H. T. Yang, and R. L. Terjung
What makes vessels grow with exercise training?
J Appl Physiol, September 1, 2004; 97(3): 1119 - 1128.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. P. Gavin and P. D. Wagner
Acute ethanol increases angiogenic growth factor gene expression in rat skeletal muscle
J Appl Physiol, March 1, 2002; 92(3): 1176 - 1182.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
I. M. Olfert, E. C. Breen, O. Mathieu-Costello, and P. D. Wagner
Skeletal muscle capillarity and angiogenic mRNA levels after exercise training in normoxia and chronic hypoxia
J Appl Physiol, September 1, 2001; 91(3): 1176 - 1184.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. B. McClelland and G. A. Brooks
Changes in MCT 1, MCT 4, and LDH expression are tissue specific in rats after long-term hypobaric hypoxia
J Appl Physiol, April 1, 2002; 92(4): 1573 - 1584.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online