| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis, Indiana 46202
Airway smooth muscle exhibits the property of length adaptation, which enables it to optimize its contractility to the mechanical conditions under which it is activated. Length adaptation has been proposed to result from a dynamic modulation of contractile and cytoskeletal filament organization, in which the cell structure adapts to changes in cell shape at different muscle lengths. Changes in filament organization would be predicted to alter muscle stiffness and extensibility. We analyzed the effects of tracheal muscle length at the time of contractile activation on the stiffness and extensibility of the muscle during subsequent stretch over a constant range of muscle lengths. Muscle strips were significantly stiffer and less extensible after contractile activation at a short length than after activation at a long length, consistent with the prediction of a shorter, thicker array of the cytoskeletal filaments at a short muscle length. Stretch beyond the length of contractile activation resulted in a persistent reduction in stiffness, suggesting a stretch-induced structural rearrangement. Our results support a model in which the filament organization of airway smooth muscle cells is plastic and can be acutely remodeled to adapt to the changes in the external physical environment.
length adaptation; deep inspiration; stretch; tidal breathing; airway responsiveness
This article has been cited by other articles:
|
|
 |
|
  N. Scichilone, A. La Sala, M. Bellia, K. Fallano, A. Togias, R. H. Brown, M. Midiri, and V. Bellia The airway response to deep inspirations decreases with COPD severity and is associated with airway distensibility assessed by computed tomography J Appl Physiol, September 1, 2008; 105(3): 832 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. LaPrad, A. R. West, P. B. Noble, K. R. Lutchen, and H. W. Mitchell Maintenance of airway caliber in isolated airways by deep inspiration and tidal strains J Appl Physiol, August 1, 2008; 105(2): 479 - 485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Tang and Y. Anfinogenova Physiologic Properties and Regulation of the Actin Cytoskeleton in Vascular Smooth Muscle Journal of Cardiovascular Pharmacology and Therapeutics, June 1, 2008; 13(2): 130 - 140. [Abstract] [PDF]
|
|
|
|
 |
|
 W. Zhang and S. J. Gunst Interactions of Airway Smooth Muscle Cells with Their Tissue Matrix: Implications for Contraction Proceedings of the ATS, January 1, 2008; 5(1): 32 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Bosse, A. Sobieszek, P. D. Pare, and C. Y. Seow Length Adaptation of Airway Smooth Muscle Proceedings of the ATS, January 1, 2008; 5(1): 62 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Eddinger and D. P. Meer Myosin II isoforms in smooth muscle: heterogeneity and function Am J Physiol Cell Physiol, August 1, 2007; 293(2): C493 - C508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hirota, P. Helli, and L. J. Janssen Ionic mechanisms and Ca2+ handling in airway smooth muscle Eur. Respir. J., July 1, 2007; 30(1): 114 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. McVicker, S.-Y. Leung, V. Kanabar, L. M. Moir, K. Mahn, K. F. Chung, and S. J. Hirst Repeated Allergen Inhalation Induces Cytoskeletal Remodeling in Smooth Muscle from Rat Bronchioles Am. J. Respir. Cell Mol. Biol., June 1, 2007; 36(6): 721 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. An, T. R. Bai, J. H. T. Bates, J. L. Black, R. H. Brown, V. Brusasco, P. Chitano, L. Deng, M. Dowell, D. H. Eidelman, et al. Airway smooth muscle dynamics: a common pathway of airway obstruction in asthma Eur. Respir. J., May 1, 2007; 29(5): 834 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. T. Bates and A.-M. Lauzon Parenchymal tethering, airway wall stiffness, and the dynamics of bronchoconstriction J Appl Physiol, May 1, 2007; 102(5): 1912 - 1920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Speich, C. Dosier, L. Borgsmiller, K. Quintero, H. P. Koo, and P. H. Ratz Adjustable passive length-tension curve in rabbit detrusor smooth muscle J Appl Physiol, May 1, 2007; 102(5): 1746 - 1755. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Speich, K. Quintero, C. Dosier, L. Borgsmiller, H. P. Koo, and P. H. Ratz A mechanical model for adjustable passive stiffness in rabbit detrusor J Appl Physiol, October 1, 2006; 101(4): 1189 - 1198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Slats, J. K. Sont, R. H.C.J. van Klink, E. H.D. Bel, and P. J. Sterk Improvement in bronchodilation following deep inspiration after a course of high-dose oral prednisone in asthma. Chest, July 1, 2006; 130(1): 58 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. An, B. Fabry, X. Trepat, N. Wang, and J. J. Fredberg Do Biophysical Properties of the Airway Smooth Muscle in Culture Predict Airway Hyperresponsiveness? Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 55 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. P. Silveira, J. P. Butler, and J. J. Fredberg Length adaptation of airway smooth muscle: a stochastic model of cytoskeletal dynamics J Appl Physiol, December 1, 2005; 99(6): 2087 - 2098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Seow Myosin filament assembly in an ever-changing myofilament lattice of smooth muscle Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1363 - C1368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Eddinger, J. D. Schiebout, and D. R. Swartz Smooth muscle adherens junctions associated proteins are stable at the cell periphery during relaxation and activation Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1379 - C1387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. T. Bates and A.-M. Lauzon Modeling the oscillation dynamics of activated airway smooth muscle strips Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L849 - L855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Speich, L. Borgsmiller, C. Call, R. Mohr, and P. H. Ratz ROK-induced cross-link formation stiffens passive muscle: reversible strain-induced stress softening in rabbit detrusor Am J Physiol Cell Physiol, July 1, 2005; 289(1): C12 - C21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Deng, N. J. Fairbank, B. Fabry, P. G. Smith, and G. N. Maksym Localized mechanical stress induces time-dependent actin cytoskeletal remodeling and stiffening in cultured airway smooth muscle cells Am J Physiol Cell Physiol, August 1, 2004; 287(2): C440 - C448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wagers, L. K. A. Lundblad, M. Ekman, C. G. Irvin, and J. H. T. Bates The allergic mouse model of asthma: normal smooth muscle in an abnormal lung? J Appl Physiol, June 1, 2004; 96(6): 2019 - 2027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. An, B. Fabry, M. Mellema, P. Bursac, W. T. Gerthoffer, U. S. Kayyali, M. Gaestel, S. A. Shore, and J. J. Fredberg Role of heat shock protein 27 in cytoskeletal remodeling of the airway smooth muscle cell J Appl Physiol, May 1, 2004; 96(5): 1701 - 1713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Opazo Saez, W. Zhang, Y. Wu, C. E. Turner, D. D. Tang, and S. J. Gunst Tension development during contractile stimulation of smooth muscle requires recruitment of paxillin and vinculin to the membrane Am J Physiol Cell Physiol, February 1, 2004; 286(2): C433 - C447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Suki and U. Frey Temporal dynamics of recurrent airway symptoms and cellular random walk J Appl Physiol, November 1, 2003; 95(5): 2122 - 2127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Tang and J. Tan Downregulation of profilin with antisense oligodeoxynucleotides inhibits force development during stimulation of smooth muscle Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1528 - H1536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Smith, L. Deng, J. J. Fredberg, and G. N. Maksym Mechanical strain increases cell stiffness through cytoskeletal filament reorganization Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L456 - L463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.G. Irvin Lung volume: a principle determinant of airway smooth muscle function Eur. Respir. J., July 1, 2003; 22(1): 3 - 5. [Full Text] [PDF]
|
|
|
|
|
|
M.A. McClean, M.J. Matheson, K. McKay, P.R.A. Johnson, A-C. Rynell, A.J. Ammit, J.L. Black, and N. Berend Low lung volume alters contractile properties of airway smooth muscle in sheep Eur. Respir. J., July 1, 2003; 22(1): 50 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Gunst and J. J. Fredberg The first three minutes: smooth muscle contraction, cytoskeletal events, and soft glasses J Appl Physiol, July 1, 2003; 95(1): 413 - 425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Smith, C. Roy, Y. N. Zhang, and S. Chauduri Mechanical Stress Increases RhoA Activation in Airway Smooth Muscle Cells Am. J. Respir. Cell Mol. Biol., April 1, 2003; 28(4): 436 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Crimi, R. Pellegrino, M. Milanese, and V. Brusasco Deep breaths, methacholine, and airway narrowing in healthy and mild asthmatic subjects J Appl Physiol, October 1, 2002; 93(4): 1384 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Waters, P. H. S. Sporn, M. Liu, and J. J. Fredberg Cellular biomechanics in the lung Am J Physiol Lung Cell Mol Physiol, September 1, 2002; 283(3): L503 - L509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Anafi and T. A. Wilson Empirical model for dynamic force-length behavior of airway smooth muscle J Appl Physiol, February 1, 2002; 92(2): 455 - 460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Gunst, X. Shen, R. Ramchandani, and R. S. Tepper Bronchoprotective and bronchodilatory effects of deep inspiration in rabbits subjected to bronchial challenge J Appl Physiol, December 1, 2001; 91(6): 2511 - 2516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Tang and S. J. Gunst Signal Transduction in Smooth Muscle: Selected Contribution: Roles of focal adhesion kinase and paxillin in the mechanosensitive regulation of myosin phosphorylation in smooth muscle J Appl Physiol, September 1, 2001; 91(3): 1452 - 1459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Seow and J. J. Fredberg Signal Transduction in Smooth Muscle: Historical perspective on airway smooth muscle: the saga of a frustrated cell J Appl Physiol, August 1, 2001; 91(2): 938 - 952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. T. Gerthoffer and S. J. Gunst Signal Transduction in Smooth Muscle: Invited Review: Focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle J Appl Physiol, August 1, 2001; 91(2): 963 - 972. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
