| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of Anesthesiology and of Physiology and Biophysics, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905
Maximum velocity of the actomyosin
ATPase reaction (Vmax ATPase) and ATP
consumption rate during maximum isometric activation (ATPiso) were determined in human vastus lateralis (VL)
muscle fibers expressing different myosin heavy chain (MHC) isoforms. We hypothesized that the reserve capacity for ATP consumption [1 
(ratio of ATPiso to Vmax
ATPase)] varies across VL muscle fibers expressing different MHC
isoforms. Biopsies were obtained from 12 subjects (10 men and 2 women;
age 21-66 yr). A quantitative histochemical procedure was used to
measure Vmax ATPase. In permeabilized fibers,
ATPiso was measured using an NADH-linked fluorometric procedure. The reserve capacity for ATP consumption was lower for
fibers coexpressing MHC2X and MHC2A compared
with fibers singularly expressing MHC2A and
MHCslow (39 vs. 52 and 56%, respectively). Tension cost
(ratio of ATPiso to generated force) also varied with fiber
type, being highest in fibers coexpressing MHC2X and MHC2A. We conclude that fiber-type differences in the
reserve capacity for ATP consumption and tension cost reflect
functional differences such as susceptibility to fatigue.
quantitative histochemistry; immunohistochemistry; muscle biopsy; sodium dodecyl sulfate-polyacrylamide electrophoresis; adenosine triphosphate
This article has been cited by other articles:
|
|
 |
|
  G. C. Sieck, W.-Z. Zhan, Y.-S. Han, and Y. S. Prakash Effect of denervation on ATP consumption rate of diaphragm muscle fibers J Appl Physiol, September 1, 2007; 103(3): 858 - 866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Howlett and M. C. Hogan Muscle: Effect of hypoxia on fatigue development in rat muscle composed of different fibre types Exp Physiol, September 1, 2007; 92(5): 887 - 894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Qian, L. W. Hunter, H. Du, Q. Ren, Y. Han, and G. C. Sieck Pkd2+/- Vascular Smooth Muscles Develop Exaggerated Vasocontraction in Response to Phenylephrine Stimulation J. Am. Soc. Nephrol., February 1, 2007; 18(2): 485 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Szentesi, M. A. Bekedam, B. J. van Beek-Harmsen, W. J. van der Laarse, R. Zaremba, A. Boonstra, F. C. Visser, and G. J. M. Stienen Depression of force production and ATPase activity in different types of human skeletal muscle fibers from patients with chronic heart failure J Appl Physiol, December 1, 2005; 99(6): 2189 - 2195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. C. Ottenheijm, L. M. A. Heunks, G. C. Sieck, W.-Z. Zhan, S. M. Jansen, H. Degens, T. de Boo, and P. N. R. Dekhuijzen Diaphragm Dysfunction in Chronic Obstructive Pulmonary Disease Am. J. Respir. Crit. Care Med., July 15, 2005; 172(2): 200 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Short, J. L. Vittone, M. L. Bigelow, D. N. Proctor, J. M. Coenen-Schimke, P. Rys, and K. S. Nair Changes in myosin heavy chain mRNA and protein expression in human skeletal muscle with age and endurance exercise training J Appl Physiol, July 1, 2005; 99(1): 95 - 102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M Gregory and C S. Bickel Recruitment Patterns in Human Skeletal Muscle During Electrical Stimulation Physical Therapy, April 1, 2005; 85(4): 358 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Levine, T. Nguyen, L. R. Kaiser, N. A. Rubinstein, G. Maislin, C. Gregory, L. C. Rome, G. A. Dudley, G. C. Sieck, and J. B. Shrager Human Diaphragm Remodeling Associated with Chronic Obstructive Pulmonary Disease: Clinical Implications Am. J. Respir. Crit. Care Med., September 15, 2003; 168(6): 706 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-S. Han, P. C. Geiger, M. J. Cody, R. L. Macken, and G. C. Sieck ATP consumption rate per cross bridge depends on myosin heavy chain isoform J Appl Physiol, June 1, 2003; 94(6): 2188 - 2196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Sieck, Y. S. Prakash, Y.-S. Han, Y.-H. Fang, P. C. Geiger, and W.-Z. Zhan Changes in actomyosin ATP consumption rate in rat diaphragm muscle fibers during postnatal development J Appl Physiol, May 1, 2003; 94(5): 1896 - 1902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C S. Bickel, J. M Slade, G. L Warren, and G. A Dudley Fatigability and Variable-Frequency Train Stimulation of Human Skeletal Muscles Physical Therapy, April 1, 2003; 83(4): 366 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Levine, C. Gregory, T. Nguyen, J. Shrager, L. Kaiser, N. Rubinstein, and G. Dudley Bioenergetic adaptation of individual human diaphragmatic myofibers to severe COPD J Appl Physiol, March 1, 2002; 92(3): 1205 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Talmadge, M. J. Castro, D. F. Apple Jr., and G. A. Dudley Phenotypic adaptations in human muscle fibers 6 and 24 wk after spinal cord injury J Appl Physiol, January 1, 2002; 92(1): 147 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. N. Proctor, J. D. Miller, N. M. Dietz, C. T. Minson, and M. J. Joyner Reduced submaximal leg blood flow after high-intensity aerobic training J Appl Physiol, December 1, 2001; 91(6): 2619 - 2627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Perrey, A. Betik, R. Candau, J. D. Rouillon, and R. L. Hughson Comparison of oxygen uptake kinetics during concentric and eccentric cycle exercise J Appl Physiol, November 1, 2001; 91(5): 2135 - 2142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Sieck and M. Regnier Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Plasticity and energetic demands of contraction in skeletal and cardiac muscle J Appl Physiol, March 1, 2001; 90(3): 1158 - 1164. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
