| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
School of Health Sciences, Deakin University, Burwood, Victoria 3125, Australia
Skeletal muscle insulin sensitivity
is enhanced after acute exercise and short-term endurance training. We
investigated the impact of exercise on the gene expression of key
insulin-signaling proteins in humans. Seven untrained subjects (4 women
and 3 men) completed 9 days of cycling at 63 ± 2% of peak
O2 uptake for 60 min/day. Muscle biopsies were taken
before, immediately after, and 3 h after the exercise bouts (on
days 1 and 9). The gene expression of insulin
receptor substrate-2 and the p85
subunit of phosphatidylinositol 3-kinase was significantly higher 3 h after a single exercise bout, although short-term training ameliorated this effect. Gene expression of insulin receptor and insulin receptor substrate-1 was not
significantly altered at any time point. These results suggest that
exercise may have a transitory impact on the expression of insulin
receptor substrate-2 and phosphatidylinositol 3-kinase; however, the
predominant actions of exercise on insulin sensitivity appear not to
reside in the transcriptional activation of the genes encoding major
insulin-signaling proteins.
messenger ribonucleic acid; real-time polymerase chain reaction; transcription; insulin-receptor substrates; phosphatidylinositol 3-kinase
This article has been cited by other articles:
|
|
 |
|
  G. D. Wadley, A. L. Siebel, G. J. Cooney, G. K. McConell, M. E. Wlodek, and J. A. Owens Uteroplacental insufficiency and reducing litter size alters skeletal muscle mitochondrial biogenesis in a sex-specific manner in the adult rat Am J Physiol Endocrinol Metab, May 1, 2008; 294(5): E861 - E869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. De Bock, W. Derave, B. O. Eijnde, M. K. Hesselink, E. Koninckx, A. J. Rose, P. Schrauwen, A. Bonen, E. A. Richter, and P. Hespel Effect of training in the fasted state on metabolic responses during exercise with carbohydrate intake J Appl Physiol, April 1, 2008; 104(4): 1045 - 1055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Wadley, J. Choate, and G. K. McConell NOS isoform-specific regulation of basal but not exercise-induced mitochondrial biogenesis in mouse skeletal muscle J. Physiol., November 15, 2007; 585(1): 253 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Wadley and G. K. McConell Effect of nitric oxide synthase inhibition on mitochondrial biogenesis in rat skeletal muscle J Appl Physiol, January 1, 2007; 102(1): 314 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Civitarese, M. K. C. Hesselink, A. P. Russell, E. Ravussin, and P. Schrauwen Glucose ingestion during exercise blunts exercise-induced gene expression of skeletal muscle fat oxidative genes Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E1023 - E1029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. N. Kraniou, D. Cameron-Smith, and M. Hargreaves Effect of short-term training on GLUT-4 mRNA and protein expression in human skeletal muscle Exp Physiol, September 1, 2004; 89(5): 559 - 563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Arkinstall, R. J. Tunstall, D. Cameron-Smith, and J. A. Hawley Regulation of metabolic genes in human skeletal muscle by short-term exercise and diet manipulation Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E25 - E31. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Spriet, R. J. Tunstall, M. J. Watt, K. A. Mehan, M. Hargreaves, and D. Cameron-Smith Pyruvate dehydrogenase activation and kinase expression in human skeletal muscle during fasting J Appl Physiol, June 1, 2004; 96(6): 2082 - 2087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. LeBlanc, S. J. Peters, R. J. Tunstall, D. Cameron-Smith, and G. J. F. Heigenhauser Effects of aerobic training on pyruvate dehydrogenase and pyruvate dehydrogenase kinase in human skeletal muscle J. Physiol., June 1, 2004; 557(2): 559 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Holten, M. Zacho, M. Gaster, C. Juel, J. F.P. Wojtaszewski, and F. Dela Strength Training Increases Insulin-Mediated Glucose Uptake, GLUT4 Content, and Insulin Signaling in Skeletal Muscle in Patients With Type 2 Diabetes Diabetes, February 1, 2004; 53(2): 294 - 305. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Cameron-Smith, L. M Burke, D. J Angus, R. J Tunstall, G. R Cox, A. Bonen, J. A Hawley, and M. Hargreaves A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle Am. J. Clinical Nutrition, February 1, 2003; 77(2): 313 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Murphy, K. K. O. Watt, D. Cameron-Smith, C. J. Gibbons, and R. J. Snow Effects of creatine supplementation on housekeeping genes in human skeletal muscle using real-time RT-PCR Physiol Genomics, January 15, 2003; 12(2): 163 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Zierath Exercise Effects of Muscle Insulin Signaling and Action: Invited Review: Exercise training-induced changes in insulin signaling in skeletal muscle J Appl Physiol, August 1, 2002; 93(2): 773 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Tunstall, K. A. Mehan, G. D. Wadley, G. R. Collier, A. Bonen, M. Hargreaves, and D. Cameron-Smith Exercise training increases lipid metabolism gene expression in human skeletal muscle Am J Physiol Endocrinol Metab, July 1, 2002; 283(1): E66 - E72. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
