| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Division of Pulmonary and Critical Care Medicine, Department of Medicine, and 3 Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44109; and 2 Department of Medicine, Medical College of Georgia, Augusta, Georgia 30912
Reactive oxygen
species contribute to diaphragm dysfunction in certain
pathophysiological conditions (i.e., sepsis and fatigue). However, the precise alterations induced by reactive oxygen species or
the specific species that are responsible for the derangements in
skeletal muscle function are incompletely understood. In this study, we
evaluated the effect of the superoxide anion radical (O2
·), hydroxyl radical (·OH), and hydrogen
peroxide (H2O2) on maximum calcium-activated
force (Fmax) and calcium sensitivity of the contractile
apparatus in chemically skinned (Triton X-100) single rat diaphragm
fibers. O2· was generated using the
xanthine/xanthine oxidase system; ·OH was generated using 1 mM
FeCl2, 1 mM ascorbate, and 1 mM
H2O2; and H2O2 was
added directly to the bathing medium. Exposure to O2· or ·OH significantly decreased
Fmax by 14.5% (P < 0.05) and 43.9%
(P < 0.005), respectively. ·OH had no effect on
Ca2+ sensitivity. Neither 10 nor 1,000 µM
H2O2 significantly altered Fmax or
Ca2+ sensitivity. We conclude that the diaphragm is
susceptible to alterations induced by a direct effect of ·OH and
O2·, but not H2O2, on the
contractile proteins, which could, in part, be responsible for
prolonged depression in contractility associated with respiratory
muscle dysfunction in certain pathophysiological conditions.
free radicals; reactive oxygen species; skinned muscle fibers; respiratory muscle; skeletal muscle
This article has been cited by other articles:
|
|
 |
|
  R. M. Murphy, T. L. Dutka, and G. D. Lamb Hydroxyl radical and glutathione interactions alter calcium sensitivity and maximum force of the contractile apparatus in rat skeletal muscle fibres J. Physiol., April 15, 2008; 586(8): 2203 - 2216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Hardin, K. S. Campbell, J. D. Smith, S. Arbogast, J. Smith, J. S. Moylan, and M. B. Reid TNF-{alpha} acts via TNFR1 and muscle-derived oxidants to depress myofibrillar force in murine skeletal muscle J Appl Physiol, March 1, 2008; 104(3): 694 - 699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Prochniewicz, D. A. Lowe, D. J. Spakowicz, L. Higgins, K. O'Conor, L. V. Thompson, D. A. Ferrington, and D. D. Thomas Functional, structural, and chemical changes in myosin associated with hydrogen peroxide treatment of skeletal muscle fibers Am J Physiol Cell Physiol, February 1, 2008; 294(2): C613 - C626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Allen, G. D. Lamb, and H. Westerblad Skeletal Muscle Fatigue: Cellular Mechanisms Physiol Rev, January 1, 2008; 88(1): 287 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Snow, N. A. Fugere, and L. V. Thompson Advanced Glycation End-Product Accumulation and Associated Protein Modification in Type II Skeletal Muscle With Aging J. Gerontol. A Biol. Sci. Med. Sci., November 1, 2007; 62(11): 1204 - 1210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. C. Ottenheijm, L. M. A. Heunks, and P. N. R. Dekhuijzen Diaphragm Muscle Fiber Dysfunction in Chronic Obstructive Pulmonary Disease: Toward a Pathophysiological Concept Am. J. Respir. Crit. Care Med., June 15, 2007; 175(12): 1233 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Supinski and L. A. Callahan Free radical-mediated skeletal muscle dysfunction in inflammatory conditions J Appl Physiol, May 1, 2007; 102(5): 2056 - 2063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamada, T. Mishima, M. Sakamoto, M. Sugiyama, S. Matsunaga, and M. Wada Myofibrillar protein oxidation and contractile dysfunction in hyperthyroid rat diaphragm J Appl Physiol, May 1, 2007; 102(5): 1850 - 1855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. van der Poel, J. N. Edwards, W. A. Macdonald, and D. G. Stephenson Mitochondrial superoxide production in skeletal muscle fibers of the rat and decreased fiber excitability Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1353 - C1360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Fugere, D. A. Ferrington, and L. V. Thompson Protein nitration with aging in the rat semimembranosus and soleus muscles. J. Gerontol. A Biol. Sci. Med. Sci., August 1, 2006; 61(8): 806 - 812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Moopanar and D. G. Allen The activity-induced reduction of myofibrillar Ca2+ sensitivity in mouse skeletal muscle is reversed by dithiothreitol J. Physiol., February 15, 2006; 571(1): 191 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. C. Ottenheijm, L. M. A. Heunks, M. C. P. Geraedts, and P. N. R. Dekhuijzen Hypoxia-induced skeletal muscle fiber dysfunction: role for reactive nitrogen species Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L127 - L135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Crouser Respiratory Failure During Critical Illness: Are Mitochondria to Blame? Am. J. Respir. Crit. Care Med., October 1, 2005; 172(7): 793 - 794. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y Warren, B. Matharoo-Ball, R. W Shaw, and R. N Khan Hydrogen peroxide and superoxide anion modulate pregnant human myometrial contractility Reproduction, October 1, 2005; 130(4): 539 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Supinski and L. A. Callahan Diaphragmatic free radical generation increases in an animal model of heart failure J Appl Physiol, September 1, 2005; 99(3): 1078 - 1084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R Moopanar and D. G Allen Reactive oxygen species reduce myofibrillar Ca2+ sensitivity in fatiguing mouse skeletal muscle at 37{degrees}C J. Physiol., April 1, 2005; 564(1): 189 - 199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G D Lamb and G S Posterino Effects of oxidation and reduction on contractile function in skeletal muscle fibres of the rat J. Physiol., January 1, 2003; 546(1): 149 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Oba, C. Kurono, R. Nakajima, T. Takaishi, K. Ishida, G. A. Fuller, W. Klomkleaw, and M. Yamaguchi H2O2 activates ryanodine receptor but has little effect on recovery of releasable Ca2+ content after fatigue J Appl Physiol, December 1, 2002; 93(6): 1999 - 2008. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
