Journal of Applied Physiology
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J Appl Physiol 90: 269-279, 2001;
8750-7587/01 $5.00
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Vol. 90, Issue 1, 269-279, January 2001

Nucleus raphé obscurus modulates hypoglossal output of neonatal rat in vitro transverse brain stem slices

John H. Peever, Aleksandar Necakov, and James Duffin

Respiratory Neuroscience Laboratory, Departments of Physiology and Anaesthesia, University of Toronto, Toronto, Ontario, Canada M5S 1A8

Nucleus raphé obscurus (NRo) modulates hypoglossal (XII) nerve motor output in the in vitro transverse brain stem slice of neonatal rats (1-5 days old); chemical ablation of NRo and its focal CO2 acidification modulated the bursting rhythm of XII nerves. We microinjected a 4.5 mM solution of kainic acid into the NRo to disrupt cellular activity and observed that XII nerve activity was temporarily abolished (n = 10). We also microinjected CO2-acidified (pH = 6.00 ± 0.01) artificial cerebrospinal fluid (aCSF) into the NRo (n = 6), the pre-Bötzinger complex (PBC) (n = 6), as well as a control region in the lateral tegmental field equidistant to NRo, PBC, and the XII motor nuclei (n = 12). CO2 acidification of the control region had no effect on XII motor output. CO2 acidification of the NRo significantly (P < 0.05) increased the burst discharge frequency of XII nerves by 77%; integrated burst amplitude and burst duration increased by 64% and 52%, respectively. CO2 acidification of the PBC significantly (P < 0.05) increased the burst discharge frequency of XII nerves by 65%, but neither integrated burst amplitude nor burst duration changed. These results demonstrate that chemical ablation of the NRo can abolish XII nerve bursting rhythm and that stimulation of the NRo with CO2-acidified aCSF can excite XII nerve bursting activity. From these observations, we conclude that, in transverse brain stem slices, the NRo contains pH/CO2-sensitive cells that modulate XII motor output.

respiratory neurons; brain stem; pH/carbon dioxide


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