Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 9: 163-170, 1956;
8750-7587/56 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huckabee, W. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huckabee, W. E.

Control of Concentration Gradients of Pyruvate and Lactate Across Cell Membranes in Blood

William E. Huckabee 1

1 From the Evans Memorial of the Massachusetts Memorial Hospitals and the Department of Medicine, Boston University School of Medicine, Boston, Massachusetts

The relationships of extracellular and intracellular concentrations of pyruvate and lactate ions were investigated using fresh human blood cells. Oxygen, CO2 and ph were constant within narrow limits because arterial blood was used, collected at –6° to –10°C and denatured in less than 3 minutes. Plasma values were derivable from whole blood concentrations by multiplying by 1.21 and 1.07, respectively, for pyruvate and lactate (per liter of plasma or blood water). Plasma/cell ratios were 2.30 and 1.37. These factors were the same at all levels of blood pyruvate and lactate during rest and exercise, fever, hyperventilation and thiamine deficiency. The values of cell membrane gradients and their behavior during completely in vivo experiments suggested that lactate moved passively, and freely, across the membrane, while the pyruvate gradient resulted from metabolic activity of the cells plus passive diffusion through a membrane of relatively high resistance.

Submitted on February 6, 1956




This article has been cited by other articles:


Home page
 ScienceHome page
A. D. Bloom and M. Tsuchioka
Lactic Dehydrogenase and Metabolism of Human Leukocytes in vitro
Science, May 19, 1967; 156(3777): 979 - 981.
[Abstract] [PDF]

Home page
 ANGIOLOGYHome page
S. A. Friedman and R. Penneys
Lactate-Pyruvate Studies in Ischemic Feet
Angiology, October 1, 1964; 15(10): 461 - 464.
[PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online