| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224; and 2 Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202
Diperoxovanadate (DPV), a potent tyrosine kinase activator and protein tyrosine phosphatase inhibitor, was utilized to explore bovine pulmonary artery endothelial cell barrier regulation. DPV produced dose-dependent decreases in transendothelial electrical resistance (TER) and increases in permeability to albumin, which were preceded by brief increases in TER (peak TER effect at 10-15 min). The significant and sustained DPV-mediated TER reductions were primarily the result of decreased intercellular resistance, rather than decreased resistance between the cell and the extracellular matrix, and were reduced by pretreatment with the tyrosine kinase inhibitor genistein but not by inhibition of p42/p44 mitogen-activating protein kinases. Immunofluorescent analysis after DPV challenge revealed dramatic F-actin polymerization and stress-fiber assembly and increased colocalization of tyrosine phosphoproteins with F-actin in a circumferential pattern at the cell periphery, changes that were abolished by genistein. The phosphorylation of focal adhesion and adherens junction proteins on tyrosine residues was confirmed in immunoprecipitates of focal adhesion kinase and cadherin-associated proteins in which dramatic dose-dependent tyrosine phosphorylation was observed after DPV stimulation. We speculate that DPV enhances endothelial cell monolayer integrity via focal adhesion plaque phosphorylation and produces subsequent monolayer destabilization of adherens junctions initiated by adherens junction protein tyrosine phosphorylation catalyzed by p60src or Src-related tyrosine kinases.
adherens junctions; cadherin; catenin; electrical resistance
This article has been cited by other articles:
|
|
 |
|
  V. Solodushko, J. C. Parker, and B. Fouty Pulmonary Microvascular Endothelial Cells Form a Tighter Monolayer when Grown in Chronic Hypoxia Am. J. Respir. Cell Mol. Biol., April 1, 2008; 38(4): 491 - 497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Troyanovsky, D. F. Alvarez, J. A. King, and K. L. Schaphorst Thrombin enhances the barrier function of rat microvascular endothelium in a PAR-1-dependent manner Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L266 - L275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Allingham, J. D. van Buul, and K. Burridge ICAM-1-Mediated, Src- and Pyk2-Dependent Vascular Endothelial Cadherin Tyrosine Phosphorylation Is Required for Leukocyte Transendothelial Migration J. Immunol., September 15, 2007; 179(6): 4053 - 4064. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Birukova, E. Alekseeva, A. Mikaelyan, and K. G. Birukov HGF attenuates thrombin-induced endothelial permeability by Tiam1-mediated activation of the Rac pathway and by Tiam1/Rac-dependent inhibition of the Rho pathway FASEB J, September 1, 2007; 21(11): 2776 - 2786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. V. Usatyuk and V. Natarajan Regulation of reactive oxygen species-induced endothelial cell-cell and cell-matrix contacts by focal adhesion kinase and adherens junction proteins Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L999 - L1010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. H. Wu Endothelial focal adhesions and barrier function J. Physiol., December 1, 2005; 569(2): 359 - 366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Verin Tyrosine Phosphorylation and Endothelial Cell Barrier Regulation Am. J. Pathol., April 1, 2005; 166(4): 955 - 957. [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kappert, K. G. Peters, F. D. Bohmer, and A. Ostman Tyrosine phosphatases in vessel wall signaling Cardiovasc Res, February 15, 2005; 65(3): 587 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-P. Lim, C. Y. Wong, L. L. Ooi, B. J. Druker, and R. J. Epstein Selective Tyrosine Hyperphosphorylation of Cytoskeletal and Stress Proteins in Primary Human Breast Cancers: Implications for Adjuvant Use of Kinase-Inhibitory Drugs Clin. Cancer Res., June 15, 2004; 10(12): 3980 - 3987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Dudek, J. R. Jacobson, E. T. Chiang, K. G. Birukov, P. Wang, X. Zhan, and J. G. N. Garcia Pulmonary Endothelial Cell Barrier Enhancement by Sphingosine 1-Phosphate: ROLES FOR CORTACTIN AND MYOSIN LIGHT CHAIN KINASE J. Biol. Chem., June 4, 2004; 279(23): 24692 - 24700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Alvarez, E.-A. B. Gjerde, and M. I. Townsley Role of EETs in regulation of endothelial permeability in rat lung Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L445 - L451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sharma, R. Sharma, E. T. McCarthy, and V. J. Savin The Focal Segmental Glomerulosclerosis Permeability Factor: Biochemical Characteristics and Biological Effects Experimental Biology and Medicine, January 1, 2004; 229(1): 85 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. V. Usatyuk, V. P. Fomin, S. Shi, J. G. N. Garcia, K. Schaphorst, and V. Natarajan Role of Ca2+ in diperoxovanadate-induced cytoskeletal remodeling and endothelial cell barrier function Am J Physiol Lung Cell Mol Physiol, November 1, 2003; 285(5): L1006 - L1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Borbiev, A. D. Verin, A. Birukova, F. Liu, M. T. Crow, and J. G. N. Garcia Role of CaM kinase II and ERK activation in thrombin-induced endothelial cell barrier dysfunction Am J Physiol Lung Cell Mol Physiol, July 1, 2003; 285(1): L43 - L54. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Young, X. Sui, T. D. Kiser, S. W. Hyun, P. Wang, S. Sakarya, D. J. Angelini, K. L. Schaphorst, J. D. Hasday, A. S. Cross, et al. Protein tyrosine phosphatase activity regulates endothelial cell-cell interactions, the paracellular pathway, and capillary tube stability Am J Physiol Lung Cell Mol Physiol, July 1, 2003; 285(1): L63 - L75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Schaphorst, E. Chiang, K. N. Jacobs, A. Zaiman, V. Natarajan, F. Wigley, and J. G. N. Garcia Role of sphingosine-1 phosphate in the enhancement of endothelial barrier integrity by platelet-released products Am J Physiol Lung Cell Mol Physiol, July 1, 2003; 285(1): L258 - L267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Birukov, A. A. Birukova, S. M. Dudek, A. D. Verin, M. T. Crow, X. Zhan, N. DePaola, and J. G. N. Garcia Shear Stress-Mediated Cytoskeletal Remodeling and Cortactin Translocation in Pulmonary Endothelial Cells Am. J. Respir. Cell Mol. Biol., April 1, 2002; 26(4): 453 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Becker, A. D. Verin, M. A. Booth, F. Liu, A. Birukova, and J. G. N. Garcia Differential regulation of diverse physiological responses to VEGF in pulmonary endothelial cells Am J Physiol Lung Cell Mol Physiol, December 1, 2001; 281(6): L1500 - L1511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Dudek and J. G. N. Garcia Cytoskeletal regulation of pulmonary vascular permeability J Appl Physiol, October 1, 2001; 91(4): 1487 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Shasby, D. R. Ries, S. S. Shasby, and M. C. Winter Histamine stimulates phosphorylation of adherens junction proteins and alters their link to vimentin Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1330 - L1338. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
