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J Appl Physiol 89: 2057-2064, 2000;
8750-7587/00 $5.00
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Vol. 89, Issue 5, 2057-2064, November 2000

Kinetics of intramuscular triglyceride fatty acids in exercising humans

Zengkui Guo, Bartolome Burguera, and Michael D. Jensen

Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota 55905

A pulse ([14C]palmitate)-chase ([3H]palmitate) approach was used to study intramuscular triglyceride (imTG) fatty acid and plasma free fatty acid (FFA) kinetics during exercise at ~45% peak O2 consumption in 12 adults. Vastus lateralis muscle was biopsied before and after 90 min of bicycle exercise; 3H2O production, breath 14CO2 excretion and lipid oxidation (indirect calorimetry) rates were measured during exercise. Results: during exercise, 8.2 ± 1.2 and 8.4 ± 0.7 µmol · kg-1 · min-1 of imTG fatty acids and plasma FFA, respectively, were oxidized according to isotopic measurements. The sum of these two values was not different (P = 0.6) from lipid oxidation by indirect calorimetry (15.4 ± 1.6 µmol · kg-1 · min-1); the isotopic and indirect calorimetry values were correlated (r = 0.79, P < 0.005). During exercise, imTG turnover rate was 0.32 ± 0.07%/min (6.0 ± 2.0 µmol of imTG · kg wet muscle-1 · min-1) and plasma FFA were incorporated into imTG at a rate of 0.7 ± 0.1 µmol · kg wet muscle-1 · min-1. The imTG pool size did not change during exercise. This pulse-chase, dual tracer appears to be a reasonable approach to measure oxidation and synthesis kinetics of imTG.

[14C]palmitate; body composition; free fatty acids


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