Journal of Applied Physiology Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 89: 1937-1942, 2000;
8750-7587/00 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (81)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chávez, J. C.
Right arrow Articles by LaManna, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chávez, J. C.
Right arrow Articles by LaManna, J. C.
Vol. 89, Issue 5, 1937-1942, November 2000

Expression of hypoxia-inducible factor-1alpha in the brain of rats during chronic hypoxia

Juan C. Chávez1, Faton Agani1, Paola Pichiule1, and Joseph C. LaManna1,2

Departments of 1 Anatomy and 2 Neurology, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106

Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that regulates adaptive responses to the lack of oxygen in mammalian cells. HIF-1 consists of two proteins, HIF-1alpha and HIF-1beta . HIF-1alpha accumulates under hypoxic conditions, whereas HIF-1beta is constitutively expressed. HIF-1alpha and HIF-1beta expression were measured during adaptation to hypobaric hypoxia (0.5 atm) in rat cerebral cortex. Western blot analyses indicated that HIF-1alpha rapidly accumulated during the onset of hypoxia and did not fall for 14 days but fell to normal by 21 days despite the continuous low arterial oxygen tension. Immunostaining showed that neurons, astrocytes, ependymal cells, and possibly endothelial cells were the cell types expressing HIF-1alpha . Genes with hypoxia-responsive elements were activated under these conditions, as evidenced by elevated vascular endothelial growth factor and glucose transporter-1 mRNA levels. When 21-day-adapted rats were exposed to a more severe hypoxic challenge (8% oxygen), HIF-1alpha accumulated again. On the basis of these results, we speculate that the vascular remodeling and metabolic changes triggered during prolonged hypoxia are capable of restoring normal tissue oxygen levels.

brain hypoxic adaptation; hypoxia-inducible genes; brain capillary angiogenesis; brain tissue oxygen; vascular endothelial growth factor


This article has been cited by other articles:


Home page
 CarcinogenesisHome page
D. A. Tennant, R. V. Duran, H. Boulahbel, and E. Gottlieb
Metabolic transformation in cancer
Carcinogenesis, August 1, 2009; 30(8): 1269 - 1280.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
O. I. Ndubuizu, J. C. Chavez, and J. C. LaManna
Increased prolyl 4-hydroxylase expression and differential regulation of hypoxia-inducible factors in the aged rat brain
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2009; 297(1): R158 - R165.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W.-Y. Chen and M.-S. Chang
IL-20 Is Regulated by Hypoxia-Inducible Factor and Up-Regulated after Experimental Ischemic Stroke
J. Immunol., April 15, 2009; 182(8): 5003 - 5012.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. El Hasnaoui-Saadani, A. Pichon, D. Marchant, P. Olivier, T. Launay, P. Quidu, M. Beaudry, A. Duvallet, J.-P. Richalet, and F. Favret
Cerebral adaptations to chronic anemia in a model of erythropoietin-deficient mice exposed to hypoxia
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2009; 296(3): R801 - R811.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
N. Schmid-Brunclik, C. Burgi-Taboada, X. Antoniou, M. Gassmann, and O. O. Ogunshola
Astrocyte responses to injury: VEGF simultaneously modulates cell death and proliferation
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2008; 295(3): R864 - R873.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. A. Qutub and A. S. Popel
Reactive Oxygen Species Regulate Hypoxia-Inducible Factor 1{alpha} Differentially in Cancer and Ischemia
Mol. Cell. Biol., August 15, 2008; 28(16): 5106 - 5119.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Neuroradiol.Home page
W.S. Bartynski
Posterior Reversible Encephalopathy Syndrome, Part 2: Controversies Surrounding Pathophysiology of Vasogenic Edema
AJNR Am. J. Neuroradiol., June 1, 2008; 29(6): 1043 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Pichiule, J. C. Chavez, A. M. Schmidt, and S. J. Vannucci
Hypoxia-inducible Factor-1 Mediates Neuronal Expression of the Receptor for Advanced Glycation End Products following Hypoxia/Ischemia
J. Biol. Chem., December 14, 2007; 282(50): 36330 - 36340.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
O. Baranova, L. F. Miranda, P. Pichiule, I. Dragatsis, R. S. Johnson, and J. C. Chavez
Neuron-Specific Inactivation of the Hypoxia Inducible Factor 1{alpha} Increases Brain Injury in a Mouse Model of Transient Focal Cerebral Ischemia
J. Neurosci., June 6, 2007; 27(23): 6320 - 6332.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
N. L. Ward, E. Moore, K. Noon, N. Spassil, E. Keenan, T. L. Ivanco, and J. C. LaManna
Cerebral angiogenic factors, angiogenesis, and physiological response to chronic hypoxia differ among four commonly used mouse strains
J Appl Physiol, May 1, 2007; 102(5): 1927 - 1935.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Y. Zhu, Y. Zhang, B. A. Ojwang, M. A. Brantley Jr, and J. M. Gidday
Long-Term Tolerance to Retinal Ischemia by Repetitive Hypoxic Preconditioning: Role of HIF-1{alpha} and Heme Oxygenase-1
Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1735 - 1743.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. M. Douglas, N. Miyasaka, K. Takahashi, A. Latuszek-Barrantes, G. G. Haddad, and H. P. Hetherington
Chronic intermittent but not constant hypoxia decreases NAA/Cr ratios in neonatal mouse hippocampus and thalamus
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1254 - R1259.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
Y.-J. Peng, G. Yuan, D. Ramakrishnan, S. D. Sharma, M. Bosch-Marce, G. K. Kumar, G. L. Semenza, and N. R. Prabhakar
Heterozygous HIF-1{alpha} deficiency impairs carotid body-mediated systemic responses and reactive oxygen species generation in mice exposed to intermittent hypoxia
J. Physiol., December 1, 2006; 577(2): 705 - 716.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. C. Chavez, O. Baranova, J. Lin, and P. Pichiule
The Transcriptional Activator Hypoxia Inducible Factor 2 (HIF-2/EPAS-1) Regulates the Oxygen-Dependent Expression of Erythropoietin in Cortical Astrocytes
J. Neurosci., September 13, 2006; 26(37): 9471 - 9481.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. W. Calvert, J. Cahill, M. Yamaguchi-Okada, and J. H. Zhang
Oxygen treatment after experimental hypoxia-ischemia in neonatal rats alters the expression of HIF-1{alpha} and its downstream target genes
J Appl Physiol, September 1, 2006; 101(3): 853 - 865.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Kanaan, R. Farahani, R. M. Douglas, J. C. LaManna, and G. G. Haddad
Effect of chronic continuous or intermittent hypoxia and reoxygenation on cerebral capillary density and myelination
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R1105 - R1114.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. Xu and J. C. LaManna
Chronic hypoxia and the cerebral circulation
J Appl Physiol, February 1, 2006; 100(2): 725 - 730.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Y. L. Ma, X. Zhu, P. M. Rivera, O. Toien, B. M. Barnes, J. C. LaManna, M. A. Smith, and K. L. Drew
Absence of cellular stress in brain after hypoxia induced by arousal from hibernation in Arctic ground squirrels
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2005; 289(5): R1297 - R1306.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Su, S. Joho, Y. Huang, A. Barcena, J. Arakawa-Hoyt, W. Grossman, and Y. W. Kan
Adeno-associated viral vector delivers cardiac-specific and hypoxia-inducible VEGF expression in ischemic mouse hearts
PNAS, November 16, 2004; 101(46): 16280 - 16285.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
C. Lundby, H. Pilegaard, J. L. Andersen, G. van Hall, M. Sander, and J. A. L. Calbet
Acclimatization to 4100 m does not change capillary density or mRNA expression of potential angiogenesis regulatory factors in human skeletal muscle
J. Exp. Biol., October 15, 2004; 207(22): 3865 - 3871.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
G. Tezel and M. B. Wax
Hypoxia-Inducible Factor 1{alpha} in the Glaucomatous Retina and Optic Nerve Head
Arch Ophthalmol, September 1, 2004; 122(9): 1348 - 1356.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
J. C. LaManna, J. C. Chavez, and P. Pichiule
Structural and functional adaptation to hypoxia in the rat brain
J. Exp. Biol., August 15, 2004; 207(18): 3163 - 3169.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
T. Acker and H. Acker
Cellular oxygen sensing need in CNS function: physiological and pathological implications
J. Exp. Biol., August 15, 2004; 207(18): 3171 - 3188.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
H. H. Marti
Erythropoietin and the hypoxic brain
J. Exp. Biol., August 15, 2004; 207(18): 3233 - 3242.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
R. A. Nordal, A. Nagy, M. Pintilie, and C. S. Wong
Hypoxia and Hypoxia-Inducible Factor-1 Target Genes in Central Nervous System Radiation Injury: A Role for Vascular Endothelial Growth Factor
Clin. Cancer Res., May 15, 2004; 10(10): 3342 - 3353.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Neuroradiol.Home page
W. S. Bartynski, Z. R. Zeigler, R. K. Shadduck, and J. Lister
Pretransplantation Conditioning Influence on the Occurrence of Cyclosporine or FK-506 Neurotoxicity in Allogeneic Bone Marrow Transplantation
AJNR Am. J. Neuroradiol., February 1, 2004; 25(2): 261 - 269.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
J. Huang, S. Z. Soffer, E. S. Kim, K. W. McCrudden, J. Huang, T. New, C. A. Manley, W. Middlesworth, K. O'Toole, D. J. Yamashiro, et al.
Vascular Remodeling Marks Tumors That Recur During Chronic Suppression of Angiogenesis
Mol. Cancer Res., January 1, 2004; 2(1): 36 - 42.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. C. Chavez and J. C. LaManna
Activation of Hypoxia-Inducible Factor-1 in the Rat Cerebral Cortex after Transient Global Ischemia: Potential Role of Insulin-Like Growth Factor-1
J. Neurosci., October 15, 2002; 22(20): 8922 - 8931.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. Pichiule and J. C. LaManna
Angiopoietin-2 and rat brain capillary remodeling during adaptation and deadaptation to prolonged mild hypoxia
J Appl Physiol, September 1, 2002; 93(3): 1131 - 1139.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. B. McClelland and G. A. Brooks
Changes in MCT 1, MCT 4, and LDH expression are tissue specific in rats after long-term hypobaric hypoxia
J Appl Physiol, April 1, 2002; 92(4): 1573 - 1584.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online