Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 89: 1065-1071, 2000;
8750-7587/00 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lan, J.
Right arrow Articles by Power, G. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lan, J.
Right arrow Articles by Power, G. G.
Vol. 89, Issue 3, 1065-1071, September 2000

Adaptation of laser-Doppler flowmetry to measure cerebral blood flow in the fetal sheep

Jiang Lan, Christian J. Hunter, Tomoaki Murata, and Gordon G. Power

Center for Perinatal Biology, School of Medicine, Loma Linda University, Loma Linda, California 92350

The purpose of this study was to devise a means to use laser-Doppler flowmetry to measure cerebral perfusion before birth. The method has not been used previously, largely because of intrauterine movement artifacts. To minimize movement artifacts, a probe holder was molded from epoxy putty to the contour of the fetal skull. A curved 18-gauge needle was embedded in the holder. At surgery, the holder, probe, and skull were fixed together with tissue glue. Residual signals were recorded after fetal death and after maternal death 1 h later. These averaged <5% of baseline flow signals, indicating minimal movement artifact. To test the usefulness of the method, cerebral flow responses were measured during moderate fetal hypoxia induced by giving the ewes ~10% oxygen in nitrogen to breathe. As fetal arterial PO2 decreased from 21.1 ± 0.5 to 10.7 ± 0.4 Torr during a 30-min period, cerebral perfusion increased progressively to 56 ± 8% above baseline. Perfusion then returned to baseline levels during a 30-min recovery period. These responses are quantitatively similar to those spot observations that have been recorded earlier using labeled microspheres. We conclude that cerebral perfusion can be successfully measured by using laser-Doppler flowmetry with the unanesthetized, chronically prepared fetal sheep as an experimental model. With this method, relative changes of perfusion from a small volume of the ovine fetal brain can be measured on a continuous basis, and movement artifacts can be reduced to 5% of measured flow values.

movement artifacts; hypoxia; probe holder


This article has been cited by other articles:


Home page
 J. Physiol.Home page
E. C. Jensen, L. Bennet, C. J. Hunter, G. C. Power, and A. J. Gunn
Post-hypoxic hypoperfusion is associated with suppression of cerebral metabolism and increased tissue oxygenation in near-term fetal sheep
J. Physiol., April 1, 2006; 572(1): 131 - 139.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
W. Pearce
Hypoxic regulation of the fetal cerebral circulation
J Appl Physiol, February 1, 2006; 100(2): 731 - 738.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
F. K. Lotgering, J. M. Bishai, P. C. Struijk, A. B. Blood, C. J. Hunter, K. C. Oberg, G. G. Power, and L. D. Longo
Absence of Robust Ischemic preconditioning by Five 1-minute total Umbilical Cord Occlusions in Fetal Sheep
Reproductive Sciences, October 1, 2004; 11(7): 449 - 456.
[Abstract] [PDF]

Home page
 StrokeHome page
C. J. Hunter, L. Bennet, G. G. Power, V. Roelfsema, A. B. Blood, J. S. Quaedackers, S. George, J. Guan, and A. J. Gunn
Key Neuroprotective Role for Endogenous Adenosine A1 Receptor Activation During Asphyxia in the Fetal Sheep
Stroke, September 1, 2003; 34(9): 2240 - 2245.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
T. Muller, M. Lohle, H. Schubert, R. Bauer, C. Wicher, I. Antonow-Schlorke, U. Sliwka, P. W. Nathanielsz, and M. Schwab
Developmental changes in cerebral autoregulatory capacity in the fetal sheep parietal cortex
J. Physiol., March 15, 2002; 539(3): 957 - 967.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online