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J Appl Physiol 89: 200-209, 2000;
8750-7587/00 $5.00
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Vol. 89, Issue 1, 200-209, July 2000

Forearm muscle metabolism studied using 31P-MRS during progressive exercise to fatigue after Acz administration

John M. Kowalchuk1,2,4, Shelly A. Smith1, Brad S. Weening1,3,4, Greg D. Marsh1,3,4, and Donald H. Paterson1,4

1 The Centre for Activity and Ageing, School of Kinesiology and Departments of 2 Physiology and 3 Medical Biophysics, University of Western Ontario, London, Ontario N6A 3K7; and 4 Lawson Research Institute, St. Joseph's Health Centre, London, Ontario, Canada N6A 4V2

The effects of acetazolamide (Acz)-induced carbonic anhydrase inhibition (CAI) on muscle intracellular thresholds (T) for intracellular pH (pHi) and inorganic phosphate-to-phosphate creatine ratio (Pi/PCr) and the plasma lactate (La-) threshold were examined in nine adult male subjects performing forearm wrist flexion exercise to fatigue. Exercise consisted of raising and lowering (1-s contraction, 1-s relaxation) a cylinder whose volume increased at a rate of 200 ml/min. The protocol was performed during control (Con) and after 45 min of CAI with Acz (10 mg/kg body wt iv). TpHi and TPi/PCr, determined using 31P-labeled magnetic resonance spectroscopy (MRS), were similar in Acz (722 ± 50 and 796 ± 75 mW, respectively) and Con (855 ± 211 and 835 ± 235 mW, respectively). The pHi was similar at end-exercise (6.38 ± 0.10 Acz and 6.43 ± 0.22 Con), but pHi recovery was slowed in Acz. In a separate experiment, blood was sampled from a deep arm vein at the elbow for determination of plasma lactate concentration ([La-]pl) and TLa-. [La-]pl was lower (P < 0.05) in Acz than Con (3.7 ± 1.7 vs. 5.0 ± 1.7 mmol/l) at end-exercise and in early recovery, but TLa- was higher (1,433 ± 243 vs. 1,041 ± 414 mW, respectively). These data suggest that the lower [La-]pl seen with CAI was not due to a delayed onset or rate of muscle La- accumulation but may be related to impaired La- removal from muscle.

intracellular threshold; acid-base; acetazolamide; lactate; intracellular pH


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