Journal of Applied Physiology Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 88: 1637-1642, 2000;
8750-7587/00 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (36)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dhanakoti, S. N.
Right arrow Articles by Raj, J. U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dhanakoti, S. N.
Right arrow Articles by Raj, J. U.
Vol. 88, Issue 5, 1637-1642, May 2000

Involvement of cGMP-dependent protein kinase in the relaxation of ovine pulmonary arteries to cGMP and cAMP

Srinivas N. Dhanakoti, Yuansheng Gao, Minh Q. Nguyen, and J. Usha Raj

Department of Pediatrics, Harbor-UCLA Medical Center, School of Medicine, University of California, Los Angeles, Torrance, California 90509

Agonist-induced smooth muscle relaxation occurs following an increase in intracellular concentrations of cGMP or cAMP. However, the role of protein kinase G (PKG) and/or protein kinase A (PKA) in cGMP- or cAMP-mediated pulmonary vasodilation is not clearly elucidated. In this study, we examined the relaxation responses of isolated pulmonary arteries of lambs (age = 10 ± 1 days), preconstricted with endothelin-1, to increasing concentrations of 8-bromo-cGMP (8-BrcGMP) or 8-BrcAMP (cell-permeable analogs), in the presence or absence of Rp-8-beta -phenyl-1,N2-etheno-bromoguanosine cyclic monosphordthioate (Rp-8-PET-BrcGMPS) or KT-5720, selective inhibitors of PKG and PKA, respectively. When examined for specificity, Rp-8-Br-PET-cGMPS abolished PKG, but not PKA, activity in pulmonary arterial extracts, whereas KT-5720 inhibited PKA activity only. 8-BrcGMP-induced relaxation was inhibited by the PKG inhibitor only, whereas 8-BrcAMP-induced relaxation was inhibited by both inhibitors. A nearly fourfold higher concentration of cAMP than cGMP was required to relax arteries by 50% and to activate PKG by 50%. Our results demonstrate that relaxation of pulmonary arteries is more sensitive to cGMP than cAMP and that PKG plays an important role in both cGMP- and cAMP-mediated relaxation.

protein kinase A; protein kinase G; lung; smooth muscle; lambs


This article has been cited by other articles:


Home page
 Ther Adv Respir DisHome page
Shu Zhu, R. E. White, and S. A. Barman
Original Research: Role of phosphodiesterases in modulation of BKCa channels in hypertensive pulmonary arterial smooth muscle
Therapeutic Advances in Respiratory Disease, June 1, 2008; 2(3): 119 - 127.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Cordaillat, A. Fort, A. Virsolvy, J.-L. Elghozi, S. Richard, and B. Jover
Nitric oxide pathway counteracts enhanced contraction to membrane depolarization in aortic rings of rats on high-sodium diet
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2007; 292(4): R1557 - R1562.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Gao, A. D. Portugal, S. Negash, W. Zhou, L. D. Longo, and J. Usha Raj
Role of Rho kinases in PKG-mediated relaxation of pulmonary arteries of fetal lambs exposed to chronic high altitude hypoxia
Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L678 - L684.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
B. O. Ibe, A. Ameer, A. M. Portugal, L. Renteria, and J. U. Raj
Platelet-Activating Factor Modulates Activity of Cyclic Nucleotides in Fetal Ovine Pulmonary Vascular Smooth Muscle
J. Pharmacol. Exp. Ther., February 1, 2007; 320(2): 728 - 737.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Worner, R. Lukowski, F. Hofmann, and J. W. Wegener
cGMP signals mainly through cAMP kinase in permeabilized murine aorta
Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H237 - H244.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. L. Kwak, K. A. Jones, D. O. Warner, and W. J. Perkins
NO responsiveness in pulmonary artery and airway smooth muscle: the role of cGMP regulation
Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L200 - L208.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
F. Sander, Y. Gao, and J. U. Raj
Hypoxia Down-Regulates Cyclic Guanidine Monophosphate-Dependent Protein Kinase in Fetal Pulmonary Vascular Smooth Muscle Cell Through Generation of Reactive Oxygen Species and Promotes Development of Pulmonary Hypertension
Chest, December 1, 2005; 128(6_suppl): 577S - 578S.
[Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
A. E. Linder, L. P. McCluskey, K. R. Cole III, K. M. Lanning, and R. C. Webb
Dynamic Association of Nitric Oxide Downstream Signaling Molecules with Endothelial Caveolin-1 in Rat Aorta
J. Pharmacol. Exp. Ther., July 1, 2005; 314(1): 9 - 15.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Gao and J. U. Raj
Role of veins in regulation of pulmonary circulation
Am J Physiol Lung Cell Mol Physiol, February 1, 2005; 288(2): L213 - L226.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
I. R. Preston, N. S. Hill, L. S. Gambardella, R. R. Warburton, and J. R. Klinger
Synergistic Effects of ANP and Sildenafil on cGMP Levels and Amelioration of Acute Hypoxic Pulmonary Hypertension
Experimental Biology and Medicine, October 1, 2004; 229(9): 920 - 925.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. A. Barman, S. Zhu, and R. E. White
PKC activates BKCa channels in rat pulmonary arterial smooth muscle via cGMP-dependent protein kinase
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1275 - L1281.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Gao, S. Dhanakoti, E. M. Trevino, X. Wang, F. C. Sander, A. D. Portugal, and J. U. Raj
Role of cGMP-dependent protein kinase in development of tolerance to nitric oxide in pulmonary veins of newborn lambs
Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L786 - L792.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
F. Li, M. De Godoy, and S. Rattan
Role of Adenylate and Guanylate Cyclases in {beta}1-, {beta}2-, and {beta}3-Adrenoceptor-Mediated Relaxation of Internal Anal Sphincter Smooth Muscle
J. Pharmacol. Exp. Ther., March 1, 2004; 308(3): 1111 - 1120.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Gao, S. Dhanakoti, E. M. Trevino, F. C. Sander, A. M. Portugal, and J. U. Raj
Effect of oxygen on cyclic GMP-dependent protein kinase-mediated relaxation in ovine fetal pulmonary arteries and veins
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L611 - L618.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. L. Jernigan, B. R. Walker, and T. C. Resta
Pulmonary PKG-1 is upregulated following chronic hypoxia
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L634 - L642.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. A. Barman, S. Zhu, G. Han, and R. E. White
cAMP activates BKCa channels in pulmonary arterial smooth muscle via cGMP-dependent protein kinase
Am J Physiol Lung Cell Mol Physiol, June 1, 2003; 284(6): L1004 - L1011.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
U. Raj and L. Shimoda
Oxygen-dependent signaling in pulmonary vascular smooth muscle
Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L671 - L677.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. D. Keef, J. R. Hume, and J. Zhong
Regulation of cardiac and smooth muscle Ca2+ channels (CaV1.2a,b) by protein kinases
Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1743 - C1756.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. A. Carvajal, K. Aguan, L. P. Thompson, I. A. Buhimschi, and C. P. Weiner
Natriuretic Peptide-Induced Relaxation of Myometrium from the Pregnant Guinea Pig Is Not Mediated by Guanylate Cyclase Activation
J. Pharmacol. Exp. Ther., April 1, 2001; 297(1): 181 - 188.
[Abstract] [Full Text]

Home page
 Exp. Biol. Med.Home page
A. A. Abdel-Latif
Cross Talk Between Cyclic Nucleotides and Polyphosphoinositide Hydrolysis, Protein Kinases, and Contraction in Smooth Muscle
Experimental Biology and Medicine, March 1, 2001; 226(3): 153 - 163.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online