| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Medical, Surgical, and Research Services, Philadelphia Veterans Affairs Medical Center, and 2 Departments of Medicine, Surgery, and Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia 19104; and 3 Department of Pediatrics, Magee-Womens Research Institute, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15213
In preliminary experiments we noted developmental (i.e., embryonic and neonatal) myosin heavy chains (MHCs) in the diaphragms of patients with severe chronic obstructive pulmonary disease (COPD). We hypothesized that this finding represented new fiber formation secondary to injury associated with the mechanical stress of COPD or previously undescribed MHCs in the human diaphragm. To distinguish between these possibilities, we analyzed diaphragmatic biopsies obtained from 9 patients with severe COPD (forced expiratory volume in 1 s = 21 ± 2% predicted, residual volume = 283 ± 22% predicted) and 10 age-matched controls. First, using immunocytochemistry with specific monoclonal antibodies, we noted that control diaphragms had greater proportions of fibers expressing embryonic (50 ± 2 vs. 28 ± 3%, P < 0.0001) and neonatal (52 ± 2 vs. 32 ± 3%, P < 0.001) MHCs than COPD diaphragms. Second, SDS-PAGE demonstrated that these developmental MHCs represented only a very small fraction of the diaphragmatic MHC content. Third, the RT-PCR demonstrated mRNA coding for embryonic and neonatal MHCs in COPD and control diaphragms. Last, COPD and control diaphragms exhibited normal histology on light microscopy. We conclude that the presence of developmental MHC isoforms does not indicate new fiber formation in diaphragms of patients with severe COPD. Although these results represent the first systematic description of embryonic and neonatal MHCs in normal adult human diaphragms, their function remains to be elucidated.
human diaphragmatic muscle; embryonic myosin heavy chain; neonatal myosin heavy chain; fiber types; immunocytochemistry
This article has been cited by other articles:
|
|
 |
|
  C. A. C. Ottenheijm, G. J. Jenniskens, M. C. P. Geraedts, T. Hafmans, L. M. A. Heunks, T. H. van Kuppevelt, and P. N. R. Dekhuijzen Diaphragm dysfunction in chronic obstructive pulmonary disease: a role for heparan sulphate? Eur. Respir. J., July 1, 2007; 30(1): 80 - 89. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. C. Ottenheijm, L. M. A. Heunks, and P. N. R. Dekhuijzen Diaphragm Muscle Fiber Dysfunction in Chronic Obstructive Pulmonary Disease: Toward a Pathophysiological Concept Am. J. Respir. Crit. Care Med., June 15, 2007; 175(12): 1233 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Levine, T. Nguyen, M. Friscia, J. Zhu, W. Szeto, J. C. Kucharczuk, B. A. Tikunov, N. A. Rubinstein, L. R. Kaiser, and J. B. Shrager Parasternal intercostal muscle remodeling in severe chronic obstructive pulmonary disease J Appl Physiol, November 1, 2006; 101(5): 1297 - 1302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nguyen, N. A. Rubinstein, C. Vijayasarathy, L. C. Rome, L. R. Kaiser, J. B. Shrager, and S. Levine Effect of chronic obstructive pulmonary disease on calcium pump ATPase expression in human diaphragm J Appl Physiol, June 1, 2005; 98(6): 2004 - 2010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.D-C. Man, J. Moxham, and M.I. Polkey Magnetic stimulation for the measurement of respiratory and skeletal muscle function Eur. Respir. J., November 1, 2004; 24(5): 846 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B Polla, G D'Antona, R Bottinelli, and C Reggiani Respiratory muscle fibres: specialisation and plasticity Thorax, September 1, 2004; 59(9): 808 - 817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.P.K.J. Engelen, M. Orozco-Levi, N.E.P. Deutz, E. Barreiro, N. Hernandez, E.F.M. Wouters, J. Gea, and A.M.W.J. Schols Glutathione and glutamate levels in the diaphragm of patients with chronic obstructive pulmonary disease Eur. Respir. J., April 1, 2004; 23(4): 545 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Levine, T. Nguyen, L. R. Kaiser, N. A. Rubinstein, G. Maislin, C. Gregory, L. C. Rome, G. A. Dudley, G. C. Sieck, and J. B. Shrager Human Diaphragm Remodeling Associated with Chronic Obstructive Pulmonary Disease: Clinical Implications Am. J. Respir. Crit. Care Med., September 15, 2003; 168(6): 706 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Ribera, B. N'Guessan, J. Zoll, D. Fortin, B. Serrurier, B. Mettauer, X. Bigard, R. Ventura-Clapier, and E. Lampert Mitochondrial Electron Transport Chain Function Is Enhanced in Inspiratory Muscles of Patients with Chronic Obstructive Pulmonary Disease Am. J. Respir. Crit. Care Med., March 15, 2003; 167(6): 873 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Levine, C. Gregory, T. Nguyen, J. Shrager, L. Kaiser, N. Rubinstein, and G. Dudley Bioenergetic adaptation of individual human diaphragmatic myofibers to severe COPD J Appl Physiol, March 1, 2002; 92(3): 1205 - 1213. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
