| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
School of Health Sciences, Deakin University, Burwood, Victoria 3125, Australia
To investigate the effect of exercise on GLUT-4, hexokinase, and glycogenin gene expression in human skeletal muscle, 10 untrained subjects (6 women and 4 men, 21.4 ± 1.2 yr, 66.3 ± 5.0 kg, peak oxygen consumption = 2.30 ± 0.19 l/min) exercised for 60 min on a cycle ergometer at a power output requiring 73 ± 4% peak oxygen consumption. Muscle samples were obtained by needle biopsy before, immediately after, and 3 h after exercise. Gene expression was quantified, relative to 29S ribosomal protein cDNA, by RT-PCR. GLUT-4 gene expression was increased immediately after exercise (1.7 ± 0.4 vs. 0.9 ± 0.3 arbitrary units; P < 0.05) and remained significantly higher than baseline 3 h after the end of exercise (2.2 ± 0.4 vs. 0.9 ± 0.3 arbitrary units; P < 0.05). Hexokinase II gene expression was significantly higher than the resting value 3 h after the end of exercise (2.9 ± 0.4 vs. 1.3 ± 0.3 arbitrary units; P < 0.05). Exercise increased glycogenin mRNA more than twofold (2.8 ± 0.6 vs. 1.2 ± 0.2 arbitrary units; P < 0.05) 3 h after the end of exercise. For the first time, we report that a single bout of exercise is sufficient to cause upregulation of GLUT-4 and glycogenin gene expression in human skeletal muscle. Whether these increases, together with the associated increase in hexokinase II gene expression, lead to increased expression of these key proteins in skeletal muscle and contribute to the enhanced skeletal muscle glucose uptake, glycogen synthesis, and insulin action observed following exercise remains to be determined.
hexokinase; glycogen; insulin action
This article has been cited by other articles:
|
|
 |
|
  H. J. Green, E. B. Bombardier, T. A. Duhamel, G. P. Holloway, A. R. Tupling, and J. Ouyang Acute responses in muscle mitochondrial and cytosolic enzyme activities during heavy intermittent exercise J Appl Physiol, April 1, 2008; 104(4): 931 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. McGee, B. J.W. van Denderen, K. F. Howlett, J. Mollica, J. D. Schertzer, B. E. Kemp, and M. Hargreaves AMP-Activated Protein Kinase Regulates GLUT4 Transcription by Phosphorylating Histone Deacetylase 5 Diabetes, April 1, 2008; 57(4): 860 - 867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Deldicque, P. Atherton, R. Patel, D. Theisen, H. Nielens, M. J. Rennie, and M. Francaux Effects of resistance exercise with and without creatine supplementation on gene expression and cell signaling in human skeletal muscle J Appl Physiol, February 1, 2008; 104(2): 371 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Green, T. A. Duhamel, G. P. Holloway, J. W. Moule, D. W. Ranney, A. R. Tupling, and J. Ouyang Rapid upregulation of GLUT-4 and MCT-4 expression during 16 h of heavy intermittent cycle exercise Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R594 - R600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Qin, Y. Oshida, P. Li, M. Kubota, M. Nagasaki, and Y. Sato Voluntary Running Improves In Vivo Insulin Resistance in High-Salt Diet Fed Rats Experimental Biology and Medicine, November 1, 2007; 232(10): 1330 - 1337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. K. McConell, S. J. Bradley, T. J. Stephens, B. J. Canny, B. A. Kingwell, and R. S. Lee-Young Skeletal muscle nNOS{micro} protein content is increased by exercise training in humans Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R821 - R828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Wilson, J. E. Gusba, D. L. Robinson, and T. E. Graham Glycogenin protein and mRNA expression in response to changing glycogen concentration in exercise and recovery Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1815 - E1822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Marchand, M. Tarnopolsky, K. B. Adamo, J. M. Bourgeois, K. Chorneyko, and T. E. Graham Quantitative assessment of human muscle glycogen granules size and number in subcellular locations during recovery from prolonged exercise J. Physiol., April 15, 2007; 580(2): 617 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. K. Trout, C. Homko, and N. C. Tkacs Methods of Measuring Insulin Sensitivity Biol Res Nurs, April 1, 2007; 8(4): 305 - 318. [Abstract] [PDF]
|
|
|
|
|
|
E. G. Churchley, V. G. Coffey, D. J. Pedersen, A. Shield, K. A. Carey, D. Cameron-Smith, and J. A. Hawley Influence of preexercise muscle glycogen content on transcriptional activity of metabolic and myogenic genes in well-trained humans J Appl Physiol, April 1, 2007; 102(4): 1604 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. N. Kraniou, D. Cameron-Smith, and M. Hargreaves Acute exercise and GLUT4 expression in human skeletal muscle: influence of exercise intensity J Appl Physiol, September 1, 2006; 101(3): 934 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Greenberg, M. J. Jurczak, A. M. Danos, and M. J. Brady Glycogen branches out: new perspectives on the role of glycogen metabolism in the integration of metabolic pathways Am J Physiol Endocrinol Metab, July 1, 2006; 291(1): E1 - E8. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Cluberton, S. L. McGee, R. M. Murphy, and M. Hargreaves Effect of carbohydrate ingestion on exercise-induced alterations in metabolic gene expression J Appl Physiol, October 1, 2005; 99(4): 1359 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shearer, T. E. Graham, D. S. Battram, D. L. Robinson, E. A. Richter, R. J. Wilson, and M. Bakovic Glycogenin activity and mRNA expression in response to volitional exhaustion in human skeletal muscle J Appl Physiol, September 1, 2005; 99(3): 957 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shearer, R. J. Wilson, D. S. Battram, E. A. Richter, D. L. Robinson, M. Bakovic, and T. E. Graham Increases in glycogenin and glycogenin mRNA accompany glycogen resynthesis in human skeletal muscle Am J Physiol Endocrinol Metab, September 1, 2005; 289(3): E508 - E514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hayashi, S. Nagasaka, N. Takahashi, I. Kusaka, S. Ishibashi, S. Numao, D. J. Lee, Y. Taki, H. Ogata, K. Tokuyama, et al. A Single Bout of Exercise at Higher Intensity Enhances Glucose Effectiveness in Sedentary Men J. Clin. Endocrinol. Metab., July 1, 2005; 90(7): 4035 - 4040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yang, A. Creer, B. Jemiolo, and S. Trappe Time course of myogenic and metabolic gene expression in response to acute exercise in human skeletal muscle J Appl Physiol, May 1, 2005; 98(5): 1745 - 1752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Sigal, G. P. Kenny, D. H. Wasserman, and C. Castaneda-Sceppa Physical Activity/Exercise and Type 2 Diabetes Diabetes Care, October 1, 2004; 27(10): 2518 - 2539. [Full Text] [PDF]
|
|
|
|
 |
|
 G. N. Kraniou, D. Cameron-Smith, and M. Hargreaves Effect of short-term training on GLUT-4 mRNA and protein expression in human skeletal muscle Exp Physiol, September 1, 2004; 89(5): 559 - 563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Arkinstall, R. J. Tunstall, D. Cameron-Smith, and J. A. Hawley Regulation of metabolic genes in human skeletal muscle by short-term exercise and diet manipulation Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E25 - E31. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. McGee and M. Hargreaves Exercise and Myocyte Enhancer Factor 2 Regulation in Human Skeletal Muscle Diabetes, May 1, 2004; 53(5): 1208 - 1214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Hildebrandt, H. Pilegaard, and P. D. Neufer Differential transcriptional activation of select metabolic genes in response to variations in exercise intensity and duration Am J Physiol Endocrinol Metab, November 1, 2003; 285(5): E1021 - E1027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. McGee, K. F. Howlett, R. L. Starkie, D. Cameron-Smith, B. E. Kemp, and M. Hargreaves Exercise Increases Nuclear AMPK {alpha}2 in Human Skeletal Muscle Diabetes, April 1, 2003; 52(4): 926 - 928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Cameron-Smith, L. M Burke, D. J Angus, R. J Tunstall, G. R Cox, A. Bonen, J. A Hawley, and M. Hargreaves A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle Am. J. Clinical Nutrition, February 1, 2003; 77(2): 313 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Tunstall, K. A. Mehan, G. D. Wadley, G. R. Collier, A. Bonen, M. Hargreaves, and D. Cameron-Smith Exercise training increases lipid metabolism gene expression in human skeletal muscle Am J Physiol Endocrinol Metab, July 1, 2002; 283(1): E66 - E72. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Hood Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Contractile activity-induced mitochondrial biogenesis in skeletal muscle J Appl Physiol, March 1, 2001; 90(3): 1137 - 1157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Wadley, R. J. Tunstall, A. Sanigorski, G. R. Collier, M. Hargreaves, and D. Cameron-Smith Differential effects of exercise on insulin-signaling gene expression in human skeletal muscle J Appl Physiol, February 1, 2001; 90(2): 436 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Schjerling, M. Hargreaves, D. Cameron-Smith, and Y. Kraniou The Importance of Internal Controls in mRNA Quantification J Appl Physiol, January 1, 2001; 90(1): 401 - 402. [Full Text] [PDF]
|
|
|
|
|
|
A. Krook, U. Widegren, X. J. Jiang, J. Henriksson, H. Wallberg-Henriksson, D. Alessi, and J. R. Zierath Effects of exercise on mitogen- and stress-activated kinase signal transduction in human skeletal muscle Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2000; 279(5): R1716 - R1721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Pilegaard, G. A. Ordway, B. Saltin, and P. D. Neufer Transcriptional regulation of gene expression in human skeletal muscle during recovery from exercise Am J Physiol Endocrinol Metab, October 1, 2000; 279(4): E806 - E814. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
