Journal of Applied Physiology AJP: Endocrinology and Metabolism
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J Appl Physiol 88: 753-760, 2000;
8750-7587/00 $5.00
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Vol. 88, Issue 2, 753-760, February 2000

Hypoxemia-induced modification of troponin I and T in canine diaphragm

Jeremy A. Simpson, Jennifer E. van Eyk, and Steve Iscoe

Department of Physiology, Queen's University, Kingston, Ontario, Canada K7L 3N6

Impaired muscle function (fatigue) may result, in part, from modification of contractile proteins due to inadequate O2 delivery. We hypothesized that severe hypoxemia would modify skeletal troponin I (TnI) and T (TnT), two regulatory contractile proteins, in respiratory muscles. Severe isocapnic hypoxemia (arterial partial pressure of O2 of ~25 Torr) in six pentobarbital sodium-anesthetized spontaneously breathing dogs increased respiratory frequency and electromyographic activity of the diaphragm and internal and external obliques, with death occurring after 131-285 min. Western blot analysis revealed proteolyis of TnI and TnT, 17.5- and 28-kDa fragments, respectively, and higher molecular mass covalent complexes, one of which (42 kDa) contained TnI (or some fragment of it) and probably TnT in the costal and crural diaphragms but not the intercostal or abdominal muscles. These modifications of myofibrillar proteins may provide a molecular basis for contractile dysfunction, including respiratory failure, under conditions of limited O2 delivery.

respiratory muscles; contractile proteins; protein degradation; respiratory failure; protein modification


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