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Division of Physiology, Department of Medicine, University of California, San Diego, La Jolla, California 92093-0623
Tissue remodeling is an adaptive response to mechanical
tension in the lung. However, the role of pulmonary
fibroblasts in this response has not been well characterized. This
study investigates the influence of extracellular matrix on the
response of fibroblasts to mechanical strain. Cells were cultured on
flexible-bottom surfaces coated with fibronectin, laminin, or elastin
and exposed to strain. Under these conditions, fibroblasts align
perpendicular to the force vector. This stimulus results in an increase
in 
1(I) procollagen mRNA in cells cultured on laminin or
elastin but not fibronectin. Increased 1(I) procollagen
mRNA was detected 6 h after exposure to strain and reached control
levels by 72 h. [3H]proline incorporation into
newly synthesized procollagen reflects changes in mRNA levels. Strained
fibroblasts cultured on laminin or elastin incorporated 190 and 114%,
respectively, more [3H]proline into procollagen
than did unstrained cells. No difference was detected in strained
fibroblasts cultured on fibronectin. These results suggest that
fibroblasts respond to mechanical strain in vitro, and this
response is signaled by cell-extracellular matrix interactions.
extracellular matrix; procollagen; remodeling
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