Journal of Applied Physiology AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 87: 1894-1900, 1999;
8750-7587/99 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Geiger, P. C.
Right arrow Articles by Sieck, G. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Geiger, P. C.
Right arrow Articles by Sieck, G. C.
Vol. 87, Issue 5, 1894-1900, November 1999

Force-calcium relationship depends on myosin heavy chain and troponin isoforms in rat diaphragm muscle fibers

Paige C. Geiger, Mark J. Cody, and Gary C. Sieck

Departments of Anesthesiology and Physiology and Biophysics, Mayo Clinic and Foundation, Rochester, Minnesota 55905

The present study examined Ca2+ sensitivity of diaphragm muscle (Diam) fibers expressing different myosin heavy chain (MHC) isoforms. We hypothesized that Diam fibers expressing the MHCslow isoform have greater Ca2+ sensitivity than fibers expressing fast MHC isoforms and that this fiber-type difference in Ca2+ sensitivity reflects the isoform composition of the troponin (Tn) complex (TnC, TnT, and TnI). Studies were performed in single Triton-X-permeabilized Diam fibers. The Ca2+ concentration at which 50% maximal force was generated (pCa50) was determined for each fiber. SDS-PAGE and Western analyses were used to determine the MHC and Tn isoform composition of single fibers. The pCa50 for Diam fibers expressing MHCslow was significantly greater than that of fibers expressing fast MHC isoforms, and this greater Ca2+ sensitivity was associated with expression of slow isoforms of the Tn complex. However, some Diam fibers expressing MHCslow contained the fast TnC isoform. These results suggest that the combination of TnT, TnI, and TnC isoforms may determine Ca2+ sensitivity in Diam fibers.

myosin heavy chain; troponin; diaphragm muscle; single fibers; calcium sensitivity; cooperativity


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
C. B. Mantilla and G. C. Sieck
Key aspects of phrenic motoneuron and diaphragm muscle development during the perinatal period
J Appl Physiol, June 1, 2008; 104(6): 1818 - 1827.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. B. Mantilla, R. V. Sill, B. Aravamudan, W.-Z. Zhan, and G. C. Sieck
Developmental effects on myonuclear domain size of rat diaphragm fibers
J Appl Physiol, March 1, 2008; 104(3): 787 - 794.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. C. Sieck, W.-Z. Zhan, Y.-S. Han, and Y. S. Prakash
Effect of denervation on ATP consumption rate of diaphragm muscle fibers
J Appl Physiol, September 1, 2007; 103(3): 858 - 866.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. W. H. van Hees, H. F. M. van der Heijden, C. A. C. Ottenheijm, L. M. A. Heunks, C. J. C. Pigmans, F. W. A. Verheugt, R. M. H. J. Brouwer, and P. N. R. Dekhuijzen
Diaphragm single-fiber weakness and loss of myosin in congestive heart failure rats
Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H819 - H828.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. Esposito, E. Germinario, M. Zanin, P. T. Palade, R. Betto, and D. Danieli-Betto
Isoform switching in myofibrillar and excitation-contraction coupling proteins contributes to diminished contractile function in regenerating rat soleus muscle
J Appl Physiol, April 1, 2007; 102(4): 1640 - 1648.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. C. Geiger, J. P. Bailey, C. B. Mantilla, W.-Z. Zhan, and G. C. Sieck
Mechanisms underlying myosin heavy chain expression during development of the rat diaphragm muscle
J Appl Physiol, December 1, 2006; 101(6): 1546 - 1555.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. Aravamudan, C. B. Mantilla, W.-Z. Zhan, and G. C. Sieck
Denervation effects on myonuclear domain size of rat diaphragm fibers
J Appl Physiol, May 1, 2006; 100(5): 1617 - 1622.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. A. C. Ottenheijm, L. M. A. Heunks, G. C. Sieck, W.-Z. Zhan, S. M. Jansen, H. Degens, T. de Boo, and P. N. R. Dekhuijzen
Diaphragm Dysfunction in Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med., July 15, 2005; 172(2): 200 - 205.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
V. J. Caiozzo, M. J. Baker, K. Huang, H. Chou, Y. Z. Wu, and K. M. Baldwin
Single-fiber myosin heavy chain polymorphism: how many patterns and what proportions?
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2003; 285(3): R570 - R580.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. C. Geiger, J. P. Bailey, W.-Z. Zhan, C. B. Mantilla, and G. C. Sieck
Denervation-induced changes in myosin heavy chain expression in the rat diaphragm muscle
J Appl Physiol, August 1, 2003; 95(2): 611 - 619.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Y.-S. Han, P. C. Geiger, M. J. Cody, R. L. Macken, and G. C. Sieck
ATP consumption rate per cross bridge depends on myosin heavy chain isoform
J Appl Physiol, June 1, 2003; 94(6): 2188 - 2196.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. C. Sieck, Y. S. Prakash, Y.-S. Han, Y.-H. Fang, P. C. Geiger, and W.-Z. Zhan
Changes in actomyosin ATP consumption rate in rat diaphragm muscle fibers during postnatal development
J Appl Physiol, May 1, 2003; 94(5): 1896 - 1902.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. B. Mantilla and G. C. Sieck
Plasticity in Respiratory Motor Control: Invited Review: Mechanisms underlying motor unit plasticity in the respiratory system
J Appl Physiol, March 1, 2003; 94(3): 1230 - 1241.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. L. Staib, S. J. Swoap, and S. K. Powers
Diaphragm contractile dysfunction in MyoD gene-inactivated mice
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2002; 283(3): R583 - R590.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. C. Geiger, M. J. Cody, R. L. Macken, M. E. Bayrd, and G. C. Sieck
Effect of unilateral denervation on maximum specific force in rat diaphragm muscle fibers
J Appl Physiol, April 1, 2001; 90(4): 1196 - 1204.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Y.-S. Han, D. N. Proctor, P. C. Geiger, and G. C. Sieck
Reserve capacity for ATP consumption during isometric contraction in human skeletal muscle fibers
J Appl Physiol, February 1, 2001; 90(2): 657 - 664.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. C. Geiger, M. J. Cody, R. L. Macken, M. E. Bayrd, Y.-H. Fang, and G. C. Sieck
Plasticity in Skeletal, Cardiac, and Smooth Muscle: Selected Contribution: Mechanisms underlying increased force generation by rat diaphragm muscle fibers during development
J Appl Physiol, January 1, 2001; 90(1): 380 - 388.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. C. Sieck and W.-Z. Zhan
Denervation alters myosin heavy chain expression and contractility of developing rat diaphragm muscle
J Appl Physiol, September 1, 2000; 89(3): 1106 - 1113.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. C. Geiger, M. J. Cody, R. L. Macken, and G. C. Sieck
Maximum specific force depends on myosin heavy chain content in rat diaphragm muscle fibers
J Appl Physiol, August 1, 2000; 89(2): 695 - 703.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. C. Geiger, M. J. Cody, Y. S. Han, L. W. Hunter, W.-Z. Zhan, and G. C. Sieck
Effects of hypothyroidism on maximum specific force in rat diaphragm muscle fibers
J Appl Physiol, April 1, 2002; 92(4): 1506 - 1514.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online