Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 87: 1266-1271, 1999;
8750-7587/99 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grasemann, H.
Right arrow Articles by De Sanctis, G. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grasemann, H.
Right arrow Articles by De Sanctis, G. T.
Vol. 87, Issue 4, 1266-1271, October 1999

INVITED REVIEW
Targeted deletion of the neutral endopeptidase gene alters ventilatory responses to acute hypoxia in mice

H. Grasemann1, B. Lu2, A. Jiao1, J. Boudreau1, N. P. Gerard2, and G. T. De Sanctis1

1 Combined Program in Pulmonary and Critical Care Medicine, Brigham and Women's Hospital and Harvard Medical School, and 2 Ina Sue Perlmutter Laboratory, Children's Hospital, Department of Medicine, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Massachusetts 02115

Neutral endopeptidase (NEP) is one of the major endopeptidases responsible for the inactivation of substance P in the carotid body, a neurotransmitter shown to be important in the transduction of hypoxic stimuli. Ventilatory responses to acute hypoxia were measured by indirect plethysmography in unanesthetized, unrestrained wild-type mice and in mice in which the NEP gene was deleted (NEP -/-). Ventilation was measured while the animals breathed room air: 12% O2 in N2 and 8% O2 in N2. Deletion of the NEP gene caused marked alterations in both the magnitude and composition of the hypoxic ventilatory response to both 8% O2 in N2 and 12% O2 in N2, compared with the wild-type mice (C57BL/6J) on the same genetic background as the NEP -/- mice. Treatment of C57BL/6J mice with thiorphan, a NEP inhibitor, resulted in a greater ventilatory response to 8% O2 because of a significantly greater shortening of expiratory time. The results of these studies demonstrate that NEP plays an important role in modifying the expression of the ventilatory response to acute hypoxia.

substance P; unanesthetized mice; control of breathing


This article has been cited by other articles:


Home page
 Ann. N. Y. Acad. Sci.Home page
N. N. NALIVAEVAA, L. FISK, E. G. KOCHKINA, S. A. PLESNEVA, I. A. ZHURAVIN, E. BABUSIKOVA, D. DOBROTA, and A. J. TURNER
Effect of Hypoxia/Ischemia and Hypoxic Preconditioning/Reperfusion on Expression of Some Amyloid-Degrading Enzymes
Ann. N.Y. Acad. Sci., December 1, 2004; 1035(1): 21 - 33.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online