Journal of Applied Physiology Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 87: 776-782, 1999;
8750-7587/99 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Supinski, G.
Right arrow Articles by DiMarco, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Supinski, G.
Right arrow Articles by DiMarco, A.
Vol. 87, Issue 2, 776-782, August 1999

Apocynin improves diaphragmatic function after endotoxin administration

G. Supinski1, D. Stofan2, D. Nethery2, L. Szweda2, and A. DiMarco2

1 Metrohealth Medical Center, Cleveland 44109; and 2 Pulmonary Division, Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106

Free radicals are known to play an important role in modulating the development of respiratory muscle dysfunction during sepsis. Moreover, neutrophil numbers increase in the diaphragm after endotoxin administration. Whether or not superoxide derived from infiltrating white blood cells contributes to muscle dysfunction during sepsis is, however, unknown. The purpose of the present study was to examine the effect of apocynin, an inhibitor of the superoxide-generating neutrophil NADPH complex, on endotoxin-induced diaphragmatic dysfunction. We studied groups of rats given saline, endotoxin, apocynin, or both endotoxin and apocynin. Animals were killed 18 h after injection, a portion of the diaphragm was used to assess force generation, and the remaining diaphragm was used for determination of 4-hydroxynonenal (a marker of lipid peroxidation) and nitrotyrosine levels (a marker of free radical-mediated protein modification). We found that endotoxin reduced diaphragm force generation and that apocynin partially prevented this decrease [e.g., force in response to 20 Hz was 23 ± 1 (SE), 12 ± 2, 23 ± 1, and 19 ± 1 N/cm2, respectively, for saline, endotoxin, apocynin, and endotoxin/apocynin groups; P < 0.001]. Apocynin also prevented endotoxin-mediated increases in diaphragm 4-hydroxynonenal and nitrotyrosine levels (P < 0.01). These data suggest that neutrophil-derived free radicals contribute to diaphragmatic dysfunction during sepsis.

free radicals; skeletal muscle; diaphragm; respiratory muscles


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
G. S. Supinski and L. A. Callahan
Free radical-mediated skeletal muscle dysfunction in inflammatory conditions
J Appl Physiol, May 1, 2007; 102(5): 2056 - 2063.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. H. Boyd, M. Divangahi, L. Yahiaoui, D. Gvozdic, S. Qureshi, and B. J. Petrof
Toll-Like Receptors Differentially Regulate CC and CXC Chemokines in Skeletal Muscle via NF-{kappa}B and Calcineurin
Infect. Immun., December 1, 2006; 74(12): 6829 - 6838.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. N. A. Hussain, G. Matar, E. Barreiro, M. Florian, M. Divangahi, and T. Vassilakopoulos
Modifications of proteins by 4-hydroxy-2-nonenal in the ventilatory muscles of rats
Am J Physiol Lung Cell Mol Physiol, May 1, 2006; 290(5): L996 - L1003.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
E. Barreiro, D. Sanchez, J. B. Galdiz, S. N. A. Hussain, J. Gea, and on behalf of the ENIGMA in COPD project
N-acetylcysteine increases manganese superoxide dismutase activity in septic rat diaphragms
Eur. Respir. J., December 1, 2005; 26(6): 1032 - 1039.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
E. Barreiro, A. S. Comtois, J. Gea, V. E. Laubach, and S. N.A. Hussain
Protein Tyrosine Nitration in the Ventilatory Muscles . Role of Nitric Oxide Synthases
Am. J. Respir. Cell Mol. Biol., April 1, 2002; 26(4): 438 - 446.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. EBIHARA, S. N. A. HUSSAIN, G. DANIALOU, W.-K. CHO, S. B. GOTTFRIED, and B. J. PETROF
Mechanical Ventilation Protects against Diaphragm Injury in Sepsis . Interaction of Oxidative and Mechanical Stresses
Am. J. Respir. Crit. Care Med., January 15, 2002; 165(2): 221 - 228.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
T.-X. JIANG, W. DARLENE REID, and J. D. ROAD
Free Radical Scavengers and Diaphragm Injury Following Inspiratory Resistive Loading
Am. J. Respir. Crit. Care Med., October 1, 2001; 164(7): 1288 - 1294.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
L. A. Callahan, D. Nethery, D. Stofan, A. DiMarco, and G. Supinski
Free Radical-Induced Contractile Protein Dysfunction in Endotoxin-Induced Sepsis
Am. J. Respir. Cell Mol. Biol., February 1, 2001; 24(2): 210 - 217.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
G. Supinski, D. Nethery, T. M. Nosek, L. A. Callahan, D. Stofan, and A. DiMarco
Endotoxin administration alters the force vs. pCa relationship of skeletal muscle fibers
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2000; 278(4): R891 - R896.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online