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Department of Exercise Sciences, University of Southern California, Los Angeles, California 90089-0652
To evaluate
the effects of endurance training in rats on fatty acid metabolism, we
measured the uptake and oxidation of palmitate in isolated rat
hindquarters as well as the content of fatty acid-binding proteins in
the plasma membranes
(FABPPM) of red and
white muscles from 16 trained (T) and 18 untrained (UT) rats.
Hindquarters were perfused with 6 mM glucose, 1,800 µM palmitate, and
[1-14C]palmitate at
rest and during electrical stimulation (ES) for 25 min.
FABPPM content was 43-226%
higher in red than in white muscles and was increased by 55% in red
muscles after training. A positive correlation was found to exist
between succinate dehydrogenase activity and
FABPPM content in muscle.
Palmitate uptake increased by 64-73% from rest to ES in both T
and UT and was 48-57% higher in T than UT both at rest (39.8 ± 3.5 vs. 26.9 ± 4.4 nmol · min
1 · g1,
T and UT, respectively) and during ES (69.0 ± 6.1 vs. 43.9 ± 4.4 nmol · min1 · g1,
T and UT, respectively). While the rats were resting, palmitate oxidation was not affected by training; palmitate oxidation during ES
was higher in T than UT rats (14.8 ± 1.3 vs. 9.3 ± 1.9 nmol · min1 · g1,
T and UT, respectively). In conclusion, endurance training increases 1) plasma free fatty acid (FFA)
uptake in resting and contracting perfused muscle,
2) plasma FFA oxidation in
contracting perfused muscle, and 3)
FABPPM content in red muscles.
These results suggest that an increased number of these putative plasma
membrane fatty acid transporters may be available in the trained muscle
and may be implicated in the regulation of plasma FFA metabolism in
skeletal muscle.
electrical stimulation; fatty acid metabolism; fatty acid transport; fatty acid uptake; hindquarter perfusion; skeletal muscle; endurance training; muscle triglycerides; fatty acid-binding proteins in plasma membranes
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