Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 86: 1866-1880, 1999;
8750-7587/99 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roerig, D. L.
Right arrow Articles by Dawson, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roerig, D. L.
Right arrow Articles by Dawson, C. A.
Vol. 86, Issue 6, 1866-1880, June 1999

Detection of changes in lung tissue properties with multiple-indicator dilution

D. L. Roerig1,4, S. H. Audi2, J. H. Linehan2, G. S. Krenz3, S. B. Ahlf4, W. Lin5, and C. A. Dawson2,4,5

1 Department of Anesthesiology and Pharmacology/Toxicology and 5 Department of Physiology, Medical College of Wisconsin, Milwaukee 53226; 2 Biomedical Engineering Department and 3 Department of Mathematics Statistics and Computer Science, Marquette University, Milwaukee 53201-1881; and 4 Department of Veterans Affairs, Zablocki Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295

We evaluated the potential utility of a group of indicators, each of which targets a particular tissue property, as indicators in the multiple-indicator dilution method to detect and to identify abnormalities in lung tissue properties resulting from lung injury models. We measured the pulmonary venous outflow concentration vs. time curves of [14C]diazepam, 3HOH, [14C]phenylethylamine, and a vascular reference indicator following their bolus injection into the pulmonary artery of isolated perfused rabbit lungs under different experimental conditions, resulting in changes in the lung tissue composition. The conditions included granulomatous inflammation, induced by the intravenous injection of complete Freund's adjuvant (CFA), and intratracheal fluid instillation, each of which resulted in similar increases in lung wet weight. Each of these conditions resulted in a unique pattern among the concentration vs. time outflow curves of the indicators studied. The patterns were quantified by using mathematical models describing the pulmonary disposition of each of the indicators studied. A unique model parameter vector was obtained for each condition, demonstrating the ability to detect and to identify changes in lung tissue properties by using the appropriate group of indicators in the multiple-indicator dilution method. One change that was particularly interesting was a CFA-induced change in the disposition of diazepam, suggestive of a substantial increase in peripheral-type benzodiazepine receptors in the inflamed lungs.

diazepam; phenylethylamine; mathematical modeling; lung inflammation; benzodiazepine receptors


This article has been cited by other articles:


Home page
 Drug Metab. Dispos.Home page
D. L. Roerig, S. H. Audi, and S. B. Ahlf
KINETIC CHARACTERIZATION OF P-GLYCOPROTEIN-MEDIATED EFFLUX OF RHODAMINE 6G IN THE INTACT RABBIT LUNG
Drug Metab. Dispos., September 1, 2004; 32(9): 953 - 958.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. H. Audi, L. E. Olson, R. D. Bongard, D. L. Roerig, M. L. Schulte, and C. A. Dawson
Toluidine blue O and methylene blue as endothelial redox probes in the intact lung
Am J Physiol Heart Circ Physiol, January 1, 2000; 278(1): H137 - H150.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. H. Audi, D. L. Roerig, S. B. Ahlf, W. Lin, and C. A. Dawson
Pulmonary inflammation alters the lung disposition of lipophilic amine indicators
J Appl Physiol, November 1, 1999; 87(5): 1831 - 1842.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online