Journal of Applied Physiology Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 86: 1702-1714, 1999;
8750-7587/99 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ingenito, E. P.
Right arrow Articles by Johnson, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ingenito, E. P.
Right arrow Articles by Johnson, M.
Vol. 86, Issue 5, 1702-1714, May 1999

Biophysical characterization and modeling of lung surfactant components

E. P. Ingenito1, L. Mark1, J. Morris2, F. F. Espinosa2, R. D. Kamm2, and M. Johnson2

1 Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, Boston 02115; and 2 Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

The present study characterizes the dynamic interfacial properties of calf lung surfactant (CLS) and samples reconstituted in a stepwise fashion from phospholipid (PL), hydrophobic apoprotein (HA), surfactant apoprotein A (SP-A), and neutral lipid fractions. Dipalmitoylphosphatidylcholine (DPPC), the major PL component of surfactant, was examined for comparison. Surface tension was measured over a range of oscillation frequencies (1-100 cycles/min) and bulk phase concentrations (0.01-1 mg/ml) by using a pulsating bubble surfactometer. Distinct differences in behavior were seen between samples. These differences were interpreted by using a previously validated model of surfactant adsorption kinetics that describes function in terms of 1) adsorption rate coefficient (k1), 2) desorption rate coefficient (k2), 3) minimum equilibrium surface tension (gamma *), 4) minimum surface tension at film collapse (gamma min), and 5) change in surface tension with interfacial area for gamma  < gamma * (m2). Results show that DPPC and PL have k1 and k2 values several orders of magnitude lower than CLS. PL had a gamma min of 19-20 dyn/cm, significantly greater than CLS (nearly zero). Addition of the HA to PL restored dynamic interfacial behavior to nearly that of CLS. However, m2 remained at a reduced level. Addition of the SP-A to PL + HA restored m2 to a level similar to that of CLS. No further improvement in function occurred with the addition of the neutral lipid. These results support prior studies that show addition of HA to the PL markedly increases adsorption and film stability. However, SP-A is required to completely normalize dynamic behavior.

surface tension; dynamic; model; surfactometer


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
S. L. Seurynck, N. J. Brown, C. W. Wu, K. W. Germino, E. K. Kohlmeir, E. P. Ingenito, M. R. Glucksberg, A. E. Barron, and M. Johnson
Optical monitoring of bubble size and shape in a pulsating bubble surfactometer
J Appl Physiol, August 1, 2005; 99(2): 624 - 633.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. Suki, S. Ito, D. Stamenovic, K. R. Lutchen, and E. P. Ingenito
Biomechanics of the lung parenchyma: critical roles of collagen and mechanical forces
J Appl Physiol, May 1, 2005; 98(5): 1892 - 1899.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. Ito, E. P. Ingenito, K. K. Brewer, L. D. Black, H. Parameswaran, K. R. Lutchen, and B. Suki
Mechanics, nonlinearity, and failure strength of lung tissue in a mouse model of emphysema: possible role of collagen remodeling
J Appl Physiol, February 1, 2005; 98(2): 503 - 511.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
W. Bernhard, P. L. Haslam, and J. Floros
From Birds to Humans: New Concepts on Airways Relative to Alveolar Surfactant
Am. J. Respir. Cell Mol. Biol., January 1, 2004; 30(1): 6 - 11.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
E. P. Ingenito, R. Mora, M. Cullivan, Y. Marzan, K. Haley, L. Mark, and L. A. Sonna
Decreased Surfactant Protein-B Expression and Surfactant Dysfunction in a Murine Model of Acute Lung Injury
Am. J. Respir. Cell Mol. Biol., July 1, 2001; 25(1): 35 - 44.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
W. Bernhard, A. Gebert, G. Vieten, G. A. Rau, J. M. Hohlfeld, A. D. Postle, and J. Freihorst
Pulmonary surfactant in birds: coping with surface tension in a tubular lung
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2001; 281(1): R327 - R337.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. P. Topulos, R. E. Brown, and J. P. Butler
Influence of lung volume on pulmonary microvascular pressure-volume characteristics
J Appl Physiol, October 1, 2000; 89(4): 1591 - 1600.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
W. BERNHARD, J. MOTTAGHIAN, A. GEBERT, G. A. RAU, H. von der HARDT, and C. F. POETS
Commercial versus Native Surfactants . Surface Activity, Molecular Components, and the Effect of Calcium
Am. J. Respir. Crit. Care Med., October 1, 2000; 162(4): 1524 - 1533.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online